Issue 20, 2018

Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Abstract

As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.

Graphical abstract: Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Supplementary files

Article information

Article type
Paper
Submitted
30 Kax 2017
Accepted
02 Cax 2018
First published
05 Cax 2018

Org. Biomol. Chem., 2018,16, 3824-3830

Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

Y. Arai, K. Yokoyama, Y. Kawahara, Q. Feng, I. Ohta, A. Shimoyama, S. Inuki, K. Fukase, K. Kabayama and Y. Fujimoto, Org. Biomol. Chem., 2018, 16, 3824 DOI: 10.1039/C7OB03205F

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