A high throughput whole cell flow cytometer screening toolbox was developed and validated by identifying improved variants (1.3–7-fold) for three hydrolases (esterase, lipase, cellulase). The screening principle is based on coupled enzymatic reaction using glucose derivatives which yield upon hydrolysis a fluorescent-hydrogel-layer on the surface of E. coli cells.
N. Lülsdorf, C. Pitzler, M. Biggel, R. Martinez, L. Vojcic and U. Schwaneberg, Chem. Commun., 2015, 51, 8679 DOI: 10.1039/C5CC01791B
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