Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

Jann Harberts; Robert Zierold; Cornelius Fendler; Aune Koitmäe; Parisa Bayat; Irene Fernandez-Cuesta; Gabriele Loers; Björn-Philipp Diercks; Ralf Fliegert; Andreas H. Guse; Carsten Ronning; Gaute Otnes; Magnus Borgström; Robert H. Blick

doi:10.1039/C8RA05320K

Culturing and patch clamping of Jurkat T cells and neurons on Al₂O₃ coated nanowire arrays of altered morphology†

Jann Harberts,^a Robert Zierold,^a Cornelius Fendler,^a Aune Koitmäe,^a Parisa Bayat,^a Irene Fernandez-Cuesta,^a Gabriele Loers,^b Björn-Philipp Diercks,^c Ralf Fliegert,^c Andreas H. Guse,^c Carsten Ronning,^d Gaute Otnes,^ef Magnus Borgström^ef and Robert H. Blick

*^ag

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* Corresponding authors

^a Center for Hybrid Nanostructures, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
E-mail: rblick@chyn.uni-hamburg.de
Tel: +49 40 42838 1975

^b Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Falkenried 94, 20251 Hamburg, Germany

^c Department of Biochemistry and Molecular Cell Biology, The Calcium Signaling Group, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20251 Hamburg, Germany

^d Institute for Solid State Physics, Friedrich-Schiller-University Jena, Helmholtzweg 3-5, 07743 Jena, Germany

^e NanoLund, Lund University, Box 118, 22100 Lund, Sweden

^f Solid State Physics, Lund University, Box 118, 22100 Lund, Sweden

^g Material Science and Engineering, College of Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA

Abstract

Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al₂O₃ coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.

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Culturing and patch clamping of Jurkat T cells and neurons on Al₂O₃ coated nanowire arrays of altered morphology†

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Culturing and patch clamping of Jurkat T cells and neurons on Al₂O₃ coated nanowire arrays of altered morphology

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