Issue 30, 2010
From the journal:

Chemical Communications

A method to site-specifically introduce methyllysine into proteins in E. coli†

Hui-wang Ai,‡a   Jae Wook Lee‡a  and  Peter G. Schultz*a  

* Corresponding authors

a Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, USA
E-mail: schultz@scripps.edu
Fax: +1 858-784-9440

Abstract

A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.

Graphical abstract: A method to site-specifically introduce methyllysine into proteins in E. coli

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Article information

DOI
https://doi.org/10.1039/C0CC00108B
Article type
Communication
Submitted
24 Feb 2010
Accepted
07 Jun 2010
First published
22 Jun 2010

Chem. Commun., 2010,46, 5506-5508

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A method to site-specifically introduce methyllysine into proteins in E. coli

H. Ai, J. W. Lee and P. G. Schultz, Chem. Commun., 2010, 46, 5506 DOI: 10.1039/C0CC00108B

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