Issue 47, 2010

Selective labeling of polypeptides using protein farnesyltransferasevia rapid oxime ligation

Abstract

An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(I)-catalyzed click reaction.

Graphical abstract: Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

Supplementary files

Article information

Article type
Communication
Submitted
17 Aug 2010
Accepted
28 Sep 2010
First published
22 Oct 2010

Chem. Commun., 2010,46, 8998-9000

Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

M. Rashidian, J. K. Dozier, S. Lenevich and M. D. Distefano, Chem. Commun., 2010, 46, 8998 DOI: 10.1039/C0CC03305G

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