Photoactivable peptides for identifying enzyme–substrate and protein–protein interactions

Dante Rotili; Mikael Altun; Refaat B. Hamed; Christoph Loenarz; Armin Thalhammer; Richard J. Hopkinson; Ya-Min Tian; Peter J. Ratcliffe; Antonello Mai; Benedikt M. Kessler; Christopher J. Schofield

doi:10.1039/C0CC04457A

Photoactivable peptides for identifying enzyme–substrate and protein–protein interactions†

Dante Rotili,^ab Mikael Altun,^c Refaat B. Hamed,‡^ad Christoph Loenarz,‡^a Armin Thalhammer,‡^a Richard J. Hopkinson,^a Ya-Min Tian,^c Peter J. Ratcliffe,^c Antonello Mai,^b Benedikt M. Kessler*^c and Christopher J. Schofield*^a

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* Corresponding authors

^a University of Oxford, Department of Chemistry and the Oxford Centre for Integrative Systems Biology, Chemistry Research Laboratory, Mansfield Road, Oxford, United Kingdom
E-mail: Christopher.schofield@chem.ox.ac.uk

^b Pasteur Institute-Cenci Bolognetti Foundation, Department of Chemistry and Technologies of Drugs, University of Rome “La Sapienza”, P.le A. Moro 5, 00185 Rome, Italy

^c Henry Wellcome Building for Molecular Physiology, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, United Kingdom
E-mail: bmk@ccmp.ox.ac.uk

^d Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Egypt

Abstract

Photoactivated cross-linking of peptides to proteins is a useful strategy for identifying enzyme–substrate and protein–protein interactions in cell lysates as demonstrated by studies on the human hypoxia inducible factor system.

Chemical Communications

Photoactivable peptides for identifying enzyme–substrate and protein–protein interactions†

Abstract

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Photoactivable peptides for identifying enzyme–substrate and protein–protein interactions

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