Issue 2, 2011
From the journal:

Chemical Communications

A novel real-time PCR method based on signaling-by-incorporation†

Da-Rae Kim,a   Hee-Chul Ahn,b   Won-Ja Leec  and  Dae-Ro Ahn*a  

* Corresponding authors

a Biomedical Research Center, Korea Institute of Science and Technology, PO Box 131, Cheongryang, Seoul 130-650, South Korea
E-mail: drahn@kist.re.kr
Fax: +822-958-5909
Tel: +822-958-6645

b Advanced Analysis Center, Korea Institute of Science and Technology, South Korea

c Division of Malaria and Parasitic Diseases, Korea Centers for Disease Control and Prevention, National Institute of Health, Seoul, South Korea

Abstract

We describe a novel real-time PCR method, which utilizes the in situ generated signal upon incorporation of a fluorogenic nucleotide. The method has been compared with a conventional method and evaluated for practical uses such as quantification of cDNA and malaria diagnosis.

Graphical abstract: A novel real-time PCR method based on signaling-by-incorporation

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Article information

DOI
https://doi.org/10.1039/C0CC04516K
Article type
Communication
Submitted
20 Oct 2010
Accepted
10 Nov 2010
First published
26 Nov 2010

Chem. Commun., 2011,47, 791-793

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A novel real-time PCR method based on signaling-by-incorporation

D. Kim, H. Ahn, W. Lee and D. Ahn, Chem. Commun., 2011, 47, 791 DOI: 10.1039/C0CC04516K

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