Issue 19, 2011
From the journal:

Chemical Communications

Fluorescence turn-on detection of a protein through the displaced single-stranded DNA bindingprotein binding to a molecular beacon†

Dan Tang,abc   Dongli Liao,ab   Qiankun Zhu,abc   Fangyuan Wang,ab   Huping Jiao,ab   Yujing Zhangc  and  Cong Yu*ab  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China
E-mail: congyu@ciac.jl.cn
Fax: +86 431-8526-2710

b Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China

c Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, P. R. China

Abstract

A new approach has been developed for the highly sensitive and selective sensing of a protein. Lysozyme binding to its aptamer prevents SSB protein binding, and the subsequent binding of the free SSBprotein to a molecular beacon results in a turn-on fluorescence signal, which can be used for lysozyme quantification.

Graphical abstract: Fluorescence turn-on detection of a protein through the displaced single-stranded DNA binding protein binding to a molecular beacon

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Article information

DOI
https://doi.org/10.1039/C1CC10316D
Article type
Communication
Submitted
17 Jan 2011
Accepted
22 Mar 2011
First published
11 Apr 2011

Chem. Commun., 2011,47, 5485-5487

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Fluorescence turn-on detection of a protein through the displaced single-stranded DNA bindingprotein binding to a molecular beacon

D. Tang, D. Liao, Q. Zhu, F. Wang, H. Jiao, Y. Zhang and C. Yu, Chem. Commun., 2011, 47, 5485 DOI: 10.1039/C1CC10316D

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