Issue 29, 2011
From the journal:

Chemical Communications

Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide†

Akiko Nomura,a   Kaori Sugizaki,a   Hiroyuki Yanagisawaa  and  Akimitsu Okamoto*ab  

* Corresponding authors

a Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan
E-mail: aki-okamoto@riken.jp
Fax: +81 48 467 9205
Tel: +81 48 467 9238

b PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

An artificial phosphopeptide recognized the difference between methylated and hydroxymethylated cytosines in DNA. The Sp1 zinc finger peptide substituted by phosphotyrosine effectively discriminated between 5-methylcytosine, 5-hydroxymethylcytosine (hmC) and unmethylated cytosine. The DNA recognition properties of the peptide differ from those of other chemicals that detect hmC.

Graphical abstract: Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide

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Article information

DOI
https://doi.org/10.1039/C1CC12131F
Article type
Communication
Submitted
14 Apr 2011
Accepted
03 Jun 2011
First published
21 Jun 2011

Chem. Commun., 2011,47, 8277-8279

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Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide

A. Nomura, K. Sugizaki, H. Yanagisawa and A. Okamoto, Chem. Commun., 2011, 47, 8277 DOI: 10.1039/C1CC12131F

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