Issue 33, 2011
From the journal:

Chemical Communications

Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe†

Jiangyan Zhang,a   Zhengping Li,*a   Hui Wang,a   Yucong Wang,a   Hongxia Jiaa  and  Jingli Yana  

* Corresponding authors

a Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, P. R. China
E-mail: lzpbd@hbu.edu.cn
Fax: +86 312 5079403

Abstract

It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity.

Graphical abstract: Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

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Article information

DOI
https://doi.org/10.1039/C1CC13466C
Article type
Communication
Submitted
12 Jun 2011
Accepted
12 Jul 2011
First published
22 Jul 2011

Chem. Commun., 2011,47, 9465-9467

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Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

J. Zhang, Z. Li, H. Wang, Y. Wang, H. Jia and J. Yan, Chem. Commun., 2011, 47, 9465 DOI: 10.1039/C1CC13466C

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