Issue 37, 2011
From the journal:

Chemical Communications

Separation of proteins using supramolecular gel electrophoresis†

Sachiyo Yamamichi,‡a   Yuki Jinno,‡a   Nana Haraya,a   Takanori Oyoshi,a   Hideyuki Tomitori,b   Keiko Kashiwagib  and  Masamichi Yamanaka*a  

* Corresponding authors

a Department of Chemistry, Faculty of Science, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
E-mail: smyaman@ipc.shizuoka.ac.jp
Fax: +81 54 237 3384
Tel: +81 54 238 4936

b Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba 288-0025, Japan

Abstract

An amphiphilic low-molecular-weight hydrogelator 1 was synthesized. A tris-glycine-SDS solution gel of 1 was applied for electrophoresis to separate proteins. Centrifugation of a mixture of protein and a hydrogel of 1 enabled the recovery of protein. Various combinations of proteins were applied for supramolecular gel electrophoresis (SUGE), and remarkably poor mobility for small proteins (<45 kDa) was found.

Graphical abstract: Separation of proteins using supramolecular gel electrophoresis

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Article information

DOI
https://doi.org/10.1039/C1CC13826J
Article type
Communication
Submitted
27 Jun 2011
Accepted
08 Aug 2011
First published
19 Aug 2011

Chem. Commun., 2011,47, 10344-10346

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Separation of proteins using supramolecular gel electrophoresis

S. Yamamichi, Y. Jinno, N. Haraya, T. Oyoshi, H. Tomitori, K. Kashiwagi and M. Yamanaka, Chem. Commun., 2011, 47, 10344 DOI: 10.1039/C1CC13826J

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