Issue 40, 2011
From the journal:

Chemical Communications

5-Hydroxymethylcytosine-selective oxidation with peroxotungstate†

Akimitsu Okamoto,*ab   Kaori Sugizaki,a   Akiko Nakamura,a   Hiroyuki Yanagisawaa  and  Shuji Ikedaa  

* Corresponding authors

a Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan
E-mail: aki-okamoto@riken.jp
Fax: +81 48 467 9205
Tel: +81 48 467 9238

b PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

Tungsten oxidation worked as a simple chemical reaction for the effective detection of 5-hydroxymethylcytosine in DNA, distinguishing it from its epigenetic precursors, 5-methylcytosine and unmethylated cytosine. The tungsten-oxidation product obtained from 5-hydroxymethylcytosine was trihydroxylated thymine and was detected as a cleavage band in gel electrophoresis after treatment with hot piperidine.

Graphical abstract: 5-Hydroxymethylcytosine-selective oxidation with peroxotungstate

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Article information

DOI
https://doi.org/10.1039/C1CC14782J
Article type
Communication
Submitted
03 Aug 2011
Accepted
19 Aug 2011
First published
15 Sep 2011

Chem. Commun., 2011,47, 11231-11233

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5-Hydroxymethylcytosine-selective oxidation with peroxotungstate

A. Okamoto, K. Sugizaki, A. Nakamura, H. Yanagisawa and S. Ikeda, Chem. Commun., 2011, 47, 11231 DOI: 10.1039/C1CC14782J

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