Issue 10, 2011

Rapid fluorescence imaging of miRNAs in human cells using templated Staudinger reaction

Abstract

It is generally accepted that microRNAs (miRNAs) play a crucial role in gene expression regulation and that their aberrant expression is intimately linked with pathologies, most notably cancer. There is thus significant interest in detecting and quantifying these important regulators. Herein, we report the fluorescence imaging of miRNAs within a few hours using a nucleic-acid templated Staudinger reaction. A good correlation between the level of miRNAs and the fluorescence intensity was observed across different cell lines. This method was shown to also be applicable for suspended cells with fluorescence quantification by flow cytometry.

Graphical abstract: Rapid fluorescence imaging of miRNAs in human cells using templated Staudinger reaction

Supplementary files

Article information

Article type
Edge Article
Submitted
04 Apr 2011
Accepted
07 Jul 2011
First published
04 Aug 2011

Chem. Sci., 2011,2, 1969-1975

Rapid fluorescence imaging of miRNAs in human cells using templated Staudinger reaction

K. Gorska, I. Keklikoglou, U. Tschulena and N. Winssinger, Chem. Sci., 2011, 2, 1969 DOI: 10.1039/C1SC00216C

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