Issue 18, 2012

Ultrasensitive electrochemical immunoassay based on graphene oxide–Ag composites for rapid determination of clenbuterol

Abstract

We report the development of an ultrasensitive amperometric biosensor based on Ag nanoparticles-decorated graphene oxide nanosheets (GO) (Ag–GO) for the rapid detection of clenbuterol (CLB). The morphology and structure of the Ag–GO labeled CLB (Ag–GO–CLB) were characterized by transmission electron microscope (TEM), atomic force microscope (AFM), and ultraviolet-visible spectroscope (UV-vis). The immunosensor was prepared by covalently immobilizing capture antibodies on a multi-walled carbon nanotubes-modified glassy carbon electrode. Through competitive immunoreactions, the Ag–GO–CLB nanocomposites were captured on the immunosensor and the silver was measured by positive differential pulse voltammetry (DPV) in KCl solution for the detection of antigen. The experimental results show a linear response over the range from 0.01 to 10.0 ng mL−1 with a lower detection limit of 6.8 pg mL−1 (signal-to-noise ratio of 3). The Ag–GO based immunosensor offers a simple and convenient route for metal-immunoassay labels, which can avoid the complicated and time-consuming dissolving of metal component for ultrasensitive determination. Moreover, the electrochemical immunoassay shows acceptable specificity and stability and is suitable for the determination of CLB in real samples.

Graphical abstract: Ultrasensitive electrochemical immunoassay based on graphene oxide–Ag composites for rapid determination of clenbuterol

Article information

Article type
Paper
Submitted
09 Apr 2012
Accepted
22 Jun 2012
First published
25 Jun 2012

Analyst, 2012,137, 4349-4355

Ultrasensitive electrochemical immunoassay based on graphene oxide–Ag composites for rapid determination of clenbuterol

J. Bai, Y. Lai, D. Jiang, Y. Zeng, Y. Xian, F. Xiao, N. Zhang, J. Hou and L. Jin, Analyst, 2012, 137, 4349 DOI: 10.1039/C2AN35473J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements