Issue 18, 2012

Protein-templated gold nanoclusters as fluorescence probes for the detection of methotrexate

Abstract

In this contribution, bovine serum albumin stabilized gold nanoclusters as novel fluorescent probes were successfully utilized for the detection of methotrexate for the first time. Our prepared gold nanoclusters exhibited strong emission with peak maximum at 633.5 nm. However, the addition of methotrexate induced the strong fluorescence intensity of the gold nanoclusters to decrease. The decrease in fluorescence intensity of the gold nanoclusters caused by methotrexate allowed the sensitive detection of methotrexate in the range of 0.0016 μg mL−1 to 24 μg mL−1. The detection limit for methotrexate is 0.9 ng mL−1 at a signal-to-noise ratio of 3. The present sensor for methotrexate detection possessed a low detection limit and wide linear range. In addition, the real samples were analyzed with satisfactory results.

Graphical abstract: Protein-templated gold nanoclusters as fluorescence probes for the detection of methotrexate

Article information

Article type
Paper
Submitted
12 Jun 2012
Accepted
05 Jul 2012
First published
06 Jul 2012

Analyst, 2012,137, 4356-4361

Protein-templated gold nanoclusters as fluorescence probes for the detection of methotrexate

Z. Chen, S. Qian, X. Chen, W. Gao and Y. Lin, Analyst, 2012, 137, 4356 DOI: 10.1039/C2AN35786K

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