Issue 59, 2012
From the journal:

Chemical Communications

Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy†

Yandong Yin,ab   Peng Wang,ab   Xin Xing Yang,ab   Xun Li,ab   Chuan Heacd  and  Xin Sheng Zhao*ab  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
E-mail: zhaoxs@pku.edu.cn

b State Key Laboratory for Structural Chemistry of Unstable and Stable Species and Biodynamic Optical Imaging Centre, Peking University, Beijing 100871, China

c Synthetic and Functional Biomolecules Centre, Peking University, Beijing 100871, China

d Department of Chemistry, The University of Chicago, Chicago IL 60637, USA

Abstract

Based on a confocal microscopy platform, we extended the FCS time window by three orders of magnitude to the s timescale by attaching a polystyrene microsphere. We simultaneously monitored the relaxations of multiple intermediates involved in DNA hairpin folding, thus offering a much more detailed view of the kinetics of hairpin folding experimentally.

Graphical abstract: Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy

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Article information

DOI
https://doi.org/10.1039/C2CC31986A
Article type
Communication
Submitted
19 Mar 2012
Accepted
06 Jun 2012
First published
08 Jun 2012

Chem. Commun., 2012,48, 7413-7415

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Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy

Y. Yin, P. Wang, X. X. Yang, X. Li, C. He and X. S. Zhao, Chem. Commun., 2012, 48, 7413 DOI: 10.1039/C2CC31986A

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