Issue 51, 2012
From the journal:

Chemical Communications

Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit†

Yong Yu,a   Jianzhao Liu,b   Zujin Zhao,b   Ka Ming Ng,a   Kathy Qian Luo*c  and  Ben Zhong Tang*b  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, HKUST, Clear Water Bay, Kowloon, Hong Kong, China

b Department of Chemistry, Institute of Molecular Functional Materials, The Hong Kong University of Science and Technology (HKUST), Kowloon, Hong Kong, China
E-mail: tangbenz@ust.hk

c Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore
E-mail: kluo@ntu.edu.sg

Abstract

A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC–dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.

Graphical abstract: Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

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Article information

DOI
https://doi.org/10.1039/C2CC32038J
Article type
Communication
Submitted
20 Mar 2012
Accepted
04 May 2012
First published
04 May 2012

Chem. Commun., 2012,48, 6360-6362

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Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

Y. Yu, J. Liu, Z. Zhao, K. M. Ng, K. Q. Luo and B. Z. Tang, Chem. Commun., 2012, 48, 6360 DOI: 10.1039/C2CC32038J

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