Issue 81, 2012
From the journal:

Chemical Communications

A label-free real time fluorometric assay for protease and inhibitor screening with a released heme†

Wenying Li,ab   Jian Chen,a   Huping Jiao,ab   Qingfeng Zhang,a   Huipeng Zhoua  and  Cong Yu*a  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China
E-mail: congyu@ciac.jl.cn
Fax: +86 431-8526-2710

b Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China

Abstract

A label free continuous assay for protease activity and inhibitor screening has been developed. A protease (trypsin) could digest hemoglobin. Free heme molecules were released. Strong π–π stacking and hydrophobic interactions with the perylene probe resulted in efficient quenching of the probe's monomer fluorescence.

Graphical abstract: A label-free real time fluorometric assay for protease and inhibitor screening with a released heme

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2CC35374A
Article type
Communication
Submitted
25 Jul 2012
Accepted
23 Aug 2012
First published
24 Aug 2012

Chem. Commun., 2012,48, 10123-10125

Permissions

Request permissions

A label-free real time fluorometric assay for protease and inhibitor screening with a released heme

W. Li, J. Chen, H. Jiao, Q. Zhang, H. Zhou and C. Yu, Chem. Commun., 2012, 48, 10123 DOI: 10.1039/C2CC35374A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements