Issue 80, 2012
From the journal:

Chemical Communications

Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme†

Tian Tian,‡a   Heng Xiao,‡a   Yuelin Long,a   Xiaoe Zhang,a   Shaoru Wang,*a   Xiang Zhou,*ab   Songmei Liuc  and  Xin Zhouc  

* Corresponding authors

a College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, P. R. China
E-mail: xzhou@whu.edu.cn, srwang@whu.edu.cn
Fax: (+)86-27-68756663

b State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, China

c Zhongnan Hospital, Wuhan University, China

Abstract

The analysis of DNA methylation and MTase (methyltransferase) activity is important in epigenetic study. We have developed a novel strategy for sensitive analysis of MTase activity based on a hairpin shaped DNAzyme; 8–17 DNAzyme amplicon has been adopted and found to be very effective in such analysis.

Graphical abstract: Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme

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Article information

DOI
https://doi.org/10.1039/C2CC35648A
Article type
Communication
Submitted
14 Jul 2012
Accepted
22 Aug 2012
First published
23 Aug 2012

Chem. Commun., 2012,48, 10031-10033

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Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme

T. Tian, H. Xiao, Y. Long, X. Zhang, S. Wang, X. Zhou, S. Liu and X. Zhou, Chem. Commun., 2012, 48, 10031 DOI: 10.1039/C2CC35648A

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