Issue 24, 2013

Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers

Abstract

Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5–S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.

Graphical abstract: Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers

Supplementary files

Article information

Article type
Communication
Submitted
13 Aug 2013
Accepted
18 Oct 2013
First published
18 Oct 2013

Analyst, 2013,138, 7294-7298

Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers

M. S. Friddin, N. P. Smithers, M. Beaugrand, I. Marcotte, P. T. F. Williamson, H. Morgan and M. R. R. de Planque, Analyst, 2013, 138, 7294 DOI: 10.1039/C3AN01540H

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