Issue 24, 2013

Cholesterol determination using protein-templated fluorescent gold nanocluster probes

Abstract

We describe the development of a fluorescent biosensor platform for soluble cholesterol based on bovine serum albumin-stabilized gold nanocluster probes co-dissolved with cholesterol oxidase (ChOx) in a surfactant emulsion system. Selective enzymatic oxidation of cholesterol to cholest-4-en-3-one by ChOx produces stoichiometric amounts of H2O2 by-product, generating a quenching response signaling the presence of cholesterol at clinically relevant levels (LOD ∼12 μM).

Graphical abstract: Cholesterol determination using protein-templated fluorescent gold nanocluster probes

Supplementary files

Article information

Article type
Communication
Submitted
15 Aug 2013
Accepted
18 Oct 2013
First published
18 Oct 2013

Analyst, 2013,138, 7299-7302

Cholesterol determination using protein-templated fluorescent gold nanocluster probes

X. Chen and G. A. Baker, Analyst, 2013, 138, 7299 DOI: 10.1039/C3AN01548C

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