Issue 23, 2014
From the journal:

Analytical Methods

On-point detection of GM rice in 20 minutes with pullulan as CPA acceleration additive†

Rui Wang,a   Jian Wu,*a   Fang Zhang,a   Liu Wanga  and  Feng Jib  

* Corresponding authors

a College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China
E-mail: wujian69@zju.edu.cn
Tel: +86-571-88982180

b The First Affiliated Hospital of College of Medicine, Zhejiang University, Hangzhou 310003, PR China

Abstract

1% (w/v) concentration of pullulan as a cross-priming isothermal amplification (CPA) reaction additive has a great acceleration effect on nucleic acid amplification. Using this property of pullulan, on-point specific detection of nucleic acid by lateral-flow dipstick (LFD) within 20 minutes was achieved with initial DNA template of 750 copies.

Graphical abstract: On-point detection of GM rice in 20 minutes with pullulan as CPA acceleration additive

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Article information

DOI
https://doi.org/10.1039/C4AY02427C
Article type
Communication
Submitted
12 Oct 2014
Accepted
14 Oct 2014
First published
22 Oct 2014

Anal. Methods, 2014,6, 9198-9201

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On-point detection of GM rice in 20 minutes with pullulan as CPA acceleration additive

R. Wang, J. Wu, F. Zhang, L. Wang and F. Ji, Anal. Methods, 2014, 6, 9198 DOI: 10.1039/C4AY02427C

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