Elucidating proline dynamics in spider dragline silk fibre using 2H–13C HETCOR MAS NMR

Abstract

²H–¹³C HETCOR MAS NMR is performed on ²H/¹³C/¹⁵N-Pro enriched A. aurantia dragline silk. Proline dynamics are extracted from ²H NMR line shapes and T₁ in a site-specific manner to elucidate the backbone and side chain molecular dynamics for the MaSp2 GPGXX β-turn regions for spider dragline silk in the dry and wet, supercontracted states.