A designer cell, surf-crs-gdh, coexpressing carbonyl reductase (crs) and glucose dehydrogenase (gdh) on the cell surface has been constructed and its enzyme activities are compared with those of the corresponding cell, cyto-crs-gdh, coexpressing crs and gdh in cytosol. For various ketones, surf-crs-gdh exhibited 48- to 265-fold higher crs activity per unit protein compared to cyto-crs-gdh.
G. Srivastava, M. Pal, S. Kaur and R. S. Jolly, Catal. Sci. Technol., 2015, 5, 105 DOI: 10.1039/C4CY01017E
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