Issue 4, 2015
From the journal:

Organic & Biomolecular Chemistry

Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract†

Atsushi Ogawa*a  and  Yasunori Doia  

* Corresponding authors

a Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan
E-mail: ogawa.atsushi.mf@ehime-u.ac.jp
Fax: +81 89 927 8450
Tel: +81 89 927 8450

Abstract

We investigated the end processing and degradation of premature tRNAs in wheat germ extract (WGE), which led to the discovery of end protectors useful for stabilizing an in vitro transcript against various ribonucleases and thereby enhancing its apparent activity in WGE.

Graphical abstract: Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract

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Article information

DOI
https://doi.org/10.1039/C4OB02221A
Article type
Communication
Submitted
20 Oct 2014
Accepted
26 Nov 2014
First published
26 Nov 2014

Org. Biomol. Chem., 2015,13, 1008-1012

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Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract

A. Ogawa and Y. Doi, Org. Biomol. Chem., 2015, 13, 1008 DOI: 10.1039/C4OB02221A

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