Issue 89, 2015

A porous hybrid imprinted membrane for selectively anchoring target proteins from a complex matrix

Abstract

A novel porous hybrid imprinted membrane (CP/CNT/DA-MIM) was prepared that could selectively anchor and separate target proteins from a complex matrix. CP/CNT/DA-MIM exhibits many of the advantages of molecularly imprinted polymers and membranes, including the high selectivity of MIPs, the lower energy consumption and the ability to continuously separate mixtures via membrane separation. The surface morphologies and physical/chemical properties of the different membranes were investigated using FTIR, XRD, DSC, XPS and SEM. The results showed that the different molecules contained within CP/CNT/DA-MIM were homogeneous; two different sizes of imprinted cavities were observed in CP/CNT/DA-MIM, which facilitate the selective anchoring property. The adsorption capacities, swelling behaviors and mechanical properties of the different constituent membranes were also compared. The results show that the adhesion and nonspecific adsorption properties of the membrane were manifestly reduced through the addition of PVP. The binding capacity and adsorption selectivity of the membrane were apparently improved because of the presence of dopamine. MWCNTs obviously improved the mechanical strength of the membranes. CP/CNT/DA-MIM was successfully applied to separate bovine serum albumin from bovine blood. CP/CNT/DA-MIM is an economical, hydrophilic and ecofriendly membrane and is a promising separation material for the large-scale continuous selective separation of target proteins from complex matrices in commercial applications.

Graphical abstract: A porous hybrid imprinted membrane for selectively anchoring target proteins from a complex matrix

Supplementary files

Article information

Article type
Paper
Submitted
21 Jun 2015
Accepted
18 Aug 2015
First published
18 Aug 2015

RSC Adv., 2015,5, 72610-72620

Author version available

A porous hybrid imprinted membrane for selectively anchoring target proteins from a complex matrix

Z. Luo, W. Du, P. Guo, P. Zheng, R. Chang, J. Wang, A. Zeng, C. Chang and Q. Fu, RSC Adv., 2015, 5, 72610 DOI: 10.1039/C5RA11966A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements