Issue 11, 2016

A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

Abstract

Bacterial infections continue to be a major cause of deaths globally, particularly in resource-poor settings. In the absence of rapid and affordable diagnostic solutions, patients are mostly administered broad spectrum antibiotics leading to antibiotics resistance and poor recovery. Culture diagnosis continues to be a gold standard for diagnosis of bacterial infection, despite its long turnaround time of 24 to 48 h. We have developed a portable immunomagnetic cell capture (iMC2) system that allows rapid culture diagnosis of bacterial pathogens. Our approach involves the culture growth of the blood samples in broth media for 6 to 8 h, followed by immunomagnetic enrichment of the target cells using the iMC2 device. The device comprises a disposable capture chip that has two chambers of 5 ml and 50 μl volume connected through a channel with a manual valve. Bacterial cells bound to antibody coated magnetic nanoparticles are swept from the 5 ml sample chamber into the 50 μl recovery chamber by moving an external magnetic field with respect to the capture chip using a linear positioner. This enables specific isolation and up to 100× enrichment of the target cells. The presence of bacteria in the recovered sample is confirmed visually using a lateral flow immunoassay. The system is demonstrated in buffer and blood samples spiked with S. typhi. The method has high sensitivity (10 CFU ml−1), specificity and a rapid turnaround time of less than 7 h, a significant improvement over conventional methods.

Graphical abstract: A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

Supplementary files

Article information

Article type
Paper
Submitted
04 Feb 2016
Accepted
07 Apr 2016
First published
07 Apr 2016

Analyst, 2016,141, 3358-3366

Author version available

A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

S. Singh, M. Upadhyay, J. Sharma, S. Gupta, P. Vivekanandan and R. Elangovan, Analyst, 2016, 141, 3358 DOI: 10.1039/C6AN00291A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements