In situ simultaneous profiling of phosphorylation and ubiquitination by single excitation-duplexed luminescence resonance energy transfer

Abstract

A dual luminescence resonance energy transfer system is developed based on aptamer-functionalized upconversion nanoparticles as the energy donor and a synthetic dye derivative as the acceptor, which can be applied in the simultaneous profiling and relative quantification of phosphorylation and ubiquitination on a specific protein, even at low levels of protein expression.