A high-performance protein crystallization plate pre-embedded with crosslinked protein microcrystals as seeds

Hai Hou; Miao Shi; Zhong-Hao Chen; Fiaz Ahmad; Yue Liu; Er-Kai Yan; Chao Luo; Jing Li; Cheng-Long Zhu; Xu-Dong Deng; Da-Chuan Yin

doi:10.1039/C8CE00807H

A high-performance protein crystallization plate pre-embedded with crosslinked protein microcrystals as seeds†

Hai Hou,

‡^ab Miao Shi,

‡*^ab Zhong-Hao Chen,^ab Fiaz Ahmad,^ab Yue Liu,^ab Er-Kai Yan,^ab Chao Luo,^ab Jing Li,^ab Cheng-Long Zhu,^ab Xu-Dong Deng*^ab and Da-Chuan Yin

*^ab

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* Corresponding authors

^a Key Laboratory for Space Bioscience & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, P. R. China
E-mail: yindc@nwpu.edu.cn, dengxd@nwpu.edu.cn

^b Shenzhen Research Institute of Northwestern Polytechnical University, Shenzhen 518057, PR China

Abstract

The wells of the novel plate are pre-embedded with cross-linked protein microcrystals which can act as the seeds for protein crystallization. The results of crystallization screening experiments showed that the novel plate exhibited dramatically improved performance in finding crystallization conditions as compared with the original CDM plate.

CrystEngComm

A high-performance protein crystallization plate pre-embedded with crosslinked protein microcrystals as seeds†

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A high-performance protein crystallization plate pre-embedded with crosslinked protein microcrystals as seeds

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