Issue 87, 2019
From the journal:

Chemical Communications

Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination†

Jingyi Wang,a   Jiachen Shang,a   Zichen Qin,a   Aijun Tong ORCID logo a  and  Yu Xiang ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China
E-mail: xiang-yu@tsinghua.edu.cn

Abstract

A fluorescence “turn-on” method for digestion-free analysis of 4-thiouridine (s4U) in nucleic acids was developed in this work based on the oxidative amination of s4U by fluoresceinamine (FAM-NH2) and periodate (IO4−). It was 125-fold more sensitive for s4U detection than the traditional UV330 absorption method, and showed excellent selectivity to s4U over 2-thiouridine (s2U) analogues and biological thiols.

Graphical abstract: Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

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Article information

DOI
https://doi.org/10.1039/C9CC06312A
Article type
Communication
Submitted
14 Aug 2019
Accepted
07 Oct 2019
First published
09 Oct 2019

Chem. Commun., 2019,55, 13096-13099

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Selective and sensitive fluorescence “turn-on” detection of 4-thiouridine in nucleic acids via oxidative amination

J. Wang, J. Shang, Z. Qin, A. Tong and Y. Xiang, Chem. Commun., 2019, 55, 13096 DOI: 10.1039/C9CC06312A

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