Issue 40, 2020
From the journal:

RSC Advances

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems†

Codruţa C. Popescu,‡a   Marius C. Stoian,‡ab   Lia-Maria Cucos, ORCID logo ‡c   Anca G. Coman,a   Antonio Radoi,b   Anca Paun,a   Niculina D. Hădade,d   Arnaud Gautier, ORCID logo e   Costin-Ioan Popescu*c  and  Mihaela Matache ORCID logo *a  

* Corresponding authors

a University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Research Centre of Applied Organic Chemistry, 90-92 Panduri Street, RO-050663 Bucharest, Romania
E-mail: mihaela.matache@g.unibuc.ro

b National Institute for Research and Development in Microtechnology – IMT Bucharest, 126A Erou Iancu Nicolae Street, Voluntari, Romania

c Institute of Biochemistry of the Romanian Academy, 296 Spl. Independentei, Bucharest, Romania
E-mail: pop@biochim.ro

d Faculty of Chemistry and Chemical Engineering, Supramolecular Organic and Organometallic Chemistry Centre, ‘‘Babes--Bolyai’’ University, 11 Arany Janos Str., RO-400028-Cluj-Napoca, Romania

e Université Clermont Auvergne, CNRS, Sigma Clermont, ICCF, F-63000 Clermont-Ferrand, France

Abstract

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.

Graphical abstract: A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D0RA02382E
Article type
Paper
Submitted
14 Mar 2020
Accepted
16 Jun 2020
First published
23 Jun 2020
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2020,10, 23931-23935

Permissions

Request permissions

A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

C. C. Popescu, M. C. Stoian, L. Cucos, A. G. Coman, A. Radoi, A. Paun, N. D. Hădade, A. Gautier, C. Popescu and M. Matache, RSC Adv., 2020, 10, 23931 DOI: 10.1039/D0RA02382E

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements