Highly sensitive detection of 6mA at single-base resolution based on A–C mismatch

Abstract

We first demonstrated that 6mA can be selectively recognized based on the selective ligation reaction of DNA ligase toward A–C mismatch and 6mA-C mismatch. This method, when further combined with amplification using RCA, achieved highly sensitive identification of 6mA in dsDNA at single-base resolution.