Design strategy for an analyte-compensated fluorescent probe to reduce its toxicity

Abstract

During biological detection, the toxicity caused by probes to living organisms is neglected. In this study, an analyte-compensated fluorescent probe (NP-SN₃) was constructed for the detection of H₂S. Through experiments with HepG2 cells and zebrafish embryos and larvae, the NP-SN₃ probe showed no significant difference in imaging performance compared with the traditional probe (NP-N₃) but exhibited lower detection-induced toxicity in the imaging of liver fibrosis in activated HSC-T6 cells. During the development of zebrafish embryos and continuous administration in rats, NP-SN₃ showed a lower death rate, higher hatchability and lower malformation in zebrafish embryos and milder pathological symptoms in stained rat tissues.