Structural characteristics and immunostimulatory activity of sea cucumber tendon polysaccharides in cyclophosphamide-induced Balb/c mice

Abstract

Sea cucumber tendon, one of the main parts of sea cucumber viscera, is widely accepted as a waste residue. In this study, a sea cucumber tendon polysaccharide (SCTPII) was purified from sea cucumber tendons and its primary structures and immunomodulatory activity were investigated. SCTPII is a triple-helix conformation homogeneous polysaccharide with a molecular weight of 3.97 × 10⁶ Da that consists of glucose and fucose with molar ratios of 92.09% and 7.91% with high thermostability. In vivo tests on immunosuppressed Balb/c mice revealed that compared with the model group, the proliferation of T cells and B cells in splenic lymphocytes of mice in the high-dose group was significantly improved by 0.92 times and 5.14 times, respectively. Additionally, SCTPII could improve the proliferation ability and phagocytosis of macrophages, as well as promoting the expression of IL-6, TNF-α and IFN-γ and enhancing the intestinal physical barrier function by increasing the protein expression of claudin-1, occludin, ZO-1 and MUC2. Furthermore, the 16S rRNA sequencing of fecal samples was performed, and gene count and α-diversity analysis revealed that SCTPs could improve the microbial community richness. In particular, SCTPs could increase the relative abundance of Lactobacillus, Bacteroides and Akkermansia and reduce the relative abundance of Lachnospiraceae_NK4A136_group and Rikenellaceae_RC9_gut_group. These results demonstrate that SCPII possesses potential immunoregulatory activities in cyclophosphamide-induced mice by regulating intestinal microbiota diversity and improving immune organs, enhancing the proliferation ability of macrophages and splenocyte proliferation, and enhancing intestinal physical barrier function, which might provide important evidence for the development and utilization of the viscera of sea cucumber.