Quantitative glycoproteomics of high-density lipoproteins

Xinyu Tang; Maurice Wong; Jennyfer Tena; Chenghao Zhu; Christopher Rhodes; Qingwen Zhou; Anita Vinjamuri; Armin Oloumi; Sucharita Boddu; Guillaume Luxardi; Emanual Maverakis; Carlito B. Lebrilla; Angela M. Zivkovic

doi:10.1039/D2RA02294J

Quantitative glycoproteomics of high-density lipoproteins†

Xinyu Tang,

^a Maurice Wong,^b Jennyfer Tena,^b Chenghao Zhu,^a Christopher Rhodes,^a Qingwen Zhou,^b Anita Vinjamuri,^b Armin Oloumi,^b Sucharita Boddu,^c Guillaume Luxardi,^c Emanual Maverakis,^c Carlito B. Lebrilla

^b and Angela M. Zivkovic*^a

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* Corresponding authors

^a Department of Nutrition, University of California, Davis, One Shields Ave., Davis, CA 9516, USA
E-mail: amzivkovic@ucdavis.edu

^b Department of Chemistry, University of California, Davis, One Shields Ave., Davis, CA 95616, USA

^c Department of Dermatology, University of California, Davis School of Medicine, 3301 C Street, Suite 1400, Sacramento, CA 95816, USA

Abstract

In this work, we developed a targeted glycoproteomic method to monitor the site-specific glycoprofiles and quantities of the most abundant HDL-associated proteins using Orbitrap LC-MS for (glyco)peptide target discovery and QqQ LC-MS for quantitative analysis. We conducted a pilot study using the workflow to determine whether HDL protein glycoprofiles are altered in healthy human participants in response to dietary glycan supplementation.

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Quantitative glycoproteomics of high-density lipoproteins†

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