Issue 34, 2023
From the journal:

Chemical Communications

Fluorescent indicator displacement assay for the discovery of UGGAA repeat-targeted small molecules†

Tomonori Shibata, ORCID logo *a   Yasumasa Matsumoto,b   Akiko Iihara,b   Kazunori Yamada,b   Hiroshi Ochiai,b   Ryo Saito,b   Shinichi Kusakab  and  Toshiyuki Kumeb  

* Corresponding authors

a Department of Regulatory Bioorganic Chemistry, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka 567-0047, Japan
E-mail: shibata@sanken.osaka-u.ac.jp

b Sohyaku. Innovative Research Division, Mitsubishi Tanabe Pharma Corporation, 1-1-1, Marunouchi, Chiyoda-ku, Tokyo 100-8205, Japan

Abstract

We report that a selective fluorescent indicator NBD-NCD for UGGAA repeats resulted in fluorescence quenching upon binding to RNA and recovered the fluorescence by displacing NBD-NCD with UGGAA repeat-targeted small molecules. The fluorescent indicator displacement assay using NBD-NCD can detect the interaction of small molecules with UGGAA repeats.

Graphical abstract: Fluorescent indicator displacement assay for the discovery of UGGAA repeat-targeted small molecules

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Article information

DOI
https://doi.org/10.1039/D3CC00837A
Article type
Communication
Submitted
22 Feb 2023
Accepted
29 Mar 2023
First published
31 Mar 2023
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2023,59, 5071-5074

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Fluorescent indicator displacement assay for the discovery of UGGAA repeat-targeted small molecules

T. Shibata, Y. Matsumoto, A. Iihara, K. Yamada, H. Ochiai, R. Saito, S. Kusaka and T. Kume, Chem. Commun., 2023, 59, 5071 DOI: 10.1039/D3CC00837A

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