A two-dimensional coordination polymer with high laccase-like activity for sensitive colorimetric detection of thiram

Abstract

In contrast to natural enzymes, nanozymes show promising advantages of low cost and high stability for analytical applications. The simple mix of L-phenylalanine (F) and Cu²⁺ produces two-dimensional nanosheets of a coordination polymer with a high surface area ratio and rich exposed active sites as a novel catalyst. As the mimetic of natural laccase, this nanozyme (F–Cu) can catalyze the oxidative coupling reaction of 2,4-dichlorophenol (2,4-DP) and 4-aminoantipyrine (4-AP) to produce a distinct red product, thus establishing an intuitive and simple method for the detection of thiram. In the range of 0–7.5 μM, the absorbance intensity was linearly related to the concentration of thiram, and the detection limit was 0.0845 μM. The F–Cu nanozyme was successfully applied to the colorimetric detection of thiram in real samples.