Development of a nanocomposite hydrogel catalyzed H2O2/TMB system for determination of chlordiazepoxide in exhaled breath condensate

Abstract

In this study, an enzyme mimic catalyzed H₂O₂–tetramethylbenzidine system based on UiO-66/Au NPs–PVA nanocomposite hydrogel was employed as an optical probe for chlordiazepoxide sensing. An excellent detection limit of 0.0032 μg mL⁻¹ with a linear range of 0.005–2.0 μg mL⁻¹ was obtained for chlordiazepoxide in exhaled breath condensate samples under optimal conditions. The validated system showed good repeatability, simplicity, and stability toward chlordiazepoxide sensing in the exhaled breath condensate of patients receiving this drug.