Surface-designed AuNPs-based fluorescent probe for ultra-sensitive detection of oral poultry antibacterial drug furaltadone via intermolecular hydrogen bonding

Abstract

Furaltadone (FTD), a nitrofuran drug, was primarily utilized as a very effective oral veterinary antibiotic, especially in poultry production farms. As a result, FTD, a form of carcinogen, might easily enter people via the food chain, leading to fatal cancers. As a result, it is critical to develop a quick and efficient approach for detecting FTD at extremely low concentrations. Considering the aforementioned purpose, pamoic acid (PA) capped gold nanoparticles (PA@AuNPs) were synthesized in spherical morphology (size 10–15 nm) using the method of chemical reduction and used as a fluorescent probe to detect FTD. The interaction between PA@AuNPs and FTD was validated by UV-vis, XRD, and FTIR methods. Microscopic images (FESEM and HRTEM) show that PA@AuNPs have varying morphologies including rod, triangle, hexagonal, and pentagonal, and average sizes of 20–50 nm after sensing FTD. The average surface roughness of PA@AuNPs was determined to be 46.75 nm using the AFM technique. The addition of FTD (0 → 100 μM) quenched the fluorescence emission intensity of PA@AuNPs at 436 nm (λ_ex 353 nm) by 4-fold. This static quenching was confirmed by the formation of a ground state complex, PA@AuNPs·FTD, between AuNPs and FTD using fluorescence lifetime analysis. The presence of an isosbestic point at 412 nm in the UV-visible titration, as well as FTIR data, further demonstrated the existence of this ground state complex. PA@AuNPs revealed high sensitivity (LoD = 9.78 nM; K_a = 1.0615 × 10² M⁻¹) to FTD in water, resulting in a decrease in predicted quantum yield (Φ_F) from 3.36% to 0.35%. To establish PA@AuNPs as a first-generation fluorescence probe for real samples, FTD in blood serum was measured (LoD = 6.07 nM; K_a = 1.0595 × 10² M⁻¹). The non-toxic cytotoxicity and bioimaging in live zebrafish broadened the practical uses of PA@AuNPs. Furthermore, the surface interactions between PA@AuNPs and FTD were studied theoretically using time-dependent density functional theory (TD-DFT) at the B3LYP/6-31G(d,p) level of theory to support the findings from the experiment.