Multimodal integrated flexible neural probe for in situ monitoring of EEG and lactic acid

Abstract

In physiological activities, the brain's electroencephalogram (EEG) signal and chemical concentration change are crucial for diagnosing and treating neurological disorders. Despite the advantages of flexible neural probes, such as their flexibility and biocompatibility, it remains a challenge to achieve in situ monitoring of electrophysiological and chemical signals on a small scale simultaneously. This study developed a new method to construct an efficient dual-sided multimodal integrated flexible neural probe, which combines a density electrode array for EEG recordings and an electrochemical sensor for detecting lactic acid. The EEG electrode array includes a 6-channel recording electrode array with each electrode 30 × 50 μm in size, and the lactic acid sensor with overall contact is approximately 100 μm wide. The EEG electrodes have an average impedance of 2.57 kΩ at 1 kHz and remained stable after immersing in NS (normal saline) for 3 months. The lactic acid sensor showed a sensitivity of 52.8 nA mM⁻¹. The in vivo experiments demonstrated that the probe can reliably monitor electrophysiological signals. The probe is able to be implanted into the desired site with the help of a guide port. This flexible neural probe can provide more comprehensive insights into brain activity in the field of neuroscience and clinical practices.