Open Access Article
This Open Access Article is licensed under a
Creative Commons Attribution 3.0 Unported Licence

Development of a red fluorescent light-up probe for highly selective and sensitive detection of vicinal dithiol-containing proteins in living cells

Yuanyuan Wang a, Xiao-Feng Yang *a, Yaogang Zhong b, Xueyun Gong a, Zheng Li b and Hua Li *ac
aKey Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, P. R. China. E-mail: xfyang@nwu.edu.cn
bCollege of Life Sciences, Northwest University, Xi'an 710069, P. R. China
cCollege of Chemistry and Chemical Engineering, Xi'an Shiyou University, Xi'an 710065, P. R. China. E-mail: huali@nwu.edu.cn

Received 1st August 2015 , Accepted 9th October 2015

First published on 23rd October 2015


Abstract

Vicinal dithiol-containing proteins (VDPs) play a key role in cellular redox homeostasis and are responsible for many diseases. Here, we develop a red fluorescent light-up probe FAsH for the highly selective and sensitive detection of VDPs using the environment-sensitive 2-(4-dimethylaminophenyl)-4-(2-carboxyphenyl)-7-diethylamino-1-benzopyrylium (F1) as the fluorescent reporter and cyclic dithiaarsane as the targeting unit. FAsH is almost nonfluorescent in aqueous solution. However, it exhibits intense fluorescence emission upon binding to reduced bovine serum albumin (rBSA, selected as the model protein). The fluorescence intensity of FAsH is directly proportional to the concentration of rBSA over the range of 0.06–0.9 μM, with a detection limit (3δ) of 0.015 μM. Importantly, the fast kinetics of binding between FAsH and VDPs (∼2.5 min) enables the dynamic tracing of VDPs in biological systems. Preliminary experiments show that FAsH can be used for the no-wash imaging of endogenous VDPs in living cells. In addition, our study shows that F1 presents both high environment-sensitivity and good fluorescence properties, and is promising for the development of no-wash fluorescent light-up probes for target-specific proteins in living cells.


Introduction

Vicinal dithiol-containing proteins (VDPs) are proteins that contain two space-closed cysteine (Cys)-sulfhydryl groups. In general, the vicinal thiols are derived from cysteines that are sequence proximal but may be formed by the juxtaposition of cysteinyl residues – present in distant segments of the same or different polypeptides.1 As the reductive end of this redox buffer network, VDPs can undergo reversible oxidative conversion to the intra- or interprotein disulfides and are highly effective in regulating the redox environment of internal cellular compartments under normal conditions.2 In addition, VDPs hold a particularly prominent position in protein synthesis and function, and are responsible for many diseases such as cancer,3 diabetes,4 stroke,5 and neurodegeneration.6 Therefore, to explore the essential roles of VDPs in cellular redox homeostasis and protein function in living cells, there is a strong desire to develop fluorescent probes for the sensitive and selective sensing of VDPs in living cells.

In recent years, two strategies have been adopted for the development of fluorescent probes for VDPs. One method employs two maleimide groups as the recognition unit, which quenches the probe's fluorescence until they both undergo thiol addition during the labeling reaction. These fluorogenic probes have been used to label vicinal thiol-containing peptides/proteins.7 However, intracellular labeling still remains a challenge for these probes since intracellular glutathione (GSH) (1–10 mM) can undergo a similar addition reaction, thus leading to a nonspecific fluorescent labeling reaction.7b Alternatively, 1,3,2-dithiarsenolane was incorporated into a variety of fluorophores to develop fluorescent probes for VDPs which have been used to identify VDPs in living cells.8 The method employs the fact that trivalent arsenicals can bind to vicinal thiol proteins with high affinities,9 whereas proteins with thiols that are not vicinal (referred to as monothiols) interact weakly with arsenicals.10 However, a potential drawback of these fluorescent probes is the strong background fluorescence from the unreacted probes inside cells which hinders the identification of labeled proteins.8a–c To circumvent this problem, a tedious washing procedure (>15 min) is required to remove the unbound probes in order to reduce the background fluorescence, which will inevitably delay the acquisition of microscopic data and thus makes the measurements prone to artifacts. To overcome this deficiency, a ratiometric fluorescent probe for VDPs was recently developed by Huang et al. based on the fluorescence resonance energy transfer (FRET) mechanism.8d Although ratiometric probes can overcome the influence of a variety of factors such as instrumental efficiency, environmental conditions and the probe concentration, the proposed probe exhibits only moderate fluorescence variations (ca. 6-fold) upon binding to VDPs. Furthermore, the probe shows a sluggish response to VDPs (>60 min), which makes it unsuitable for the real time monitoring of VDPs inside living cells. Therefore, it is highly desirable to develop fluorescence turn-on probes for intracellular VDPs that have the combination of high selectivity, fast labeling rates, and high fluorescent turn-on ratios.

Recently, a simple strategy for designing fluorescence turn-on probes for selective protein detection has been developed by taking advantage of environment-sensitive fluorophores,11 which generally involves the incorporation of an environment-sensitive fluorophore into a ligand specific to the target protein. Typically, these probes exhibit very weak fluorescence in polar and protic environments, while the fluorescence is enhanced when the environment becomes hydrophobic or less polar. They could provide a fluorogenic response to their immediate environment, resulting in a variety of applications in bioanalytical chemistry. This light-up strategy has paved a new way for the detection of targeting proteins with high sensitivity and selectivity.

Although several environment-sensitive fluorophores have been reported,12 they show some crucial drawbacks. First, most of those used for light-up probe design are limited to those with blue or green emission, whereas far-red and near-infrared (NIR) dyes are advantageous for cellular studies due to lower photodamage, light scattering, and autofluorescence in living systems.13 Second, sensitivity to solvent polarity of these dyes is frequently not enough to detect subtle changes in the environment of the biomolecule of interest. Finally, polarity-sensitive dyes with red emission generally exhibit relatively lower sensitivity to polarity compared to blue dyes.12b Therefore, currently intensive research is focusing on the design of environment-sensitive fluorophores with red-shifted emission.

Herein, we report the design and synthesis of a red fluorescent light-up probe for the rapid detection of VDPs both in vitro and in vivo with excellent sensitivity and specificity. In the proposed sensing system, 2-(4-dimethylaminophenyl)-4-(2-carboxyphenyl)-7-diethylamino-1-benzopyrylium (F1) was selected as the environment-sensitive fluorescence reporter and cyclic dithiaarsane as the specific ligand for VDPs. Our rationale is depicted in Scheme 1. We envisioned that the selective binding of protein vicinal dithiols to the trivalent arsenical of FAsH would bring the fluorophore into the hydrophobic protein domain, and the hydrophobic environment would cause the fluorophore to emit strong fluorescence. In contrast, in the absence of the target protein, the probe would remain in aqueous solution and should emit only weak fluorescence. Based on the above mechanism, we created a selective fluorescence turn-on probe toward VDPs inside living cells with no-wash procedures. Compared with the reported fluorescent probes, FAsH is cell-permeable and shows a rapid response toward VDPs with high sensitivity. Moreover, the proposed probe can operate in the red region, which is favorable for biological applications in vitro and in vivo. The proposed probe has been used for rapid no-wash imaging of VDPs in living cells.


image file: c5sc02824h-s1.tif
Scheme 1 (a) Proposed reaction between FAsH and rBSA; (b) schematic illustration of the fluorescence turn-on mechanism for rBSA detection with FAsH.

Results and discussion

Probe design

In this work, we constructed a novel fluorescent light-up probe for the selective detection of VDPs inside living cells by adopting a fluorogenic mechanism based on an environment-sensitive fluorophore. To obtain an optimal response, there is a strong need to select environment-sensitive dyes presenting both high environment-sensitivity and good fluorescence properties. In this contribution, we focused our interest on a flavylium fluorescent dye F1, because it can be regarded as an electron acceptor system (benzopyrylium cation) connected to two different electron donor units (dimethylaminophenyl and diethylamino groups) (Fig. 1), which is a typical characteristic of representative solvatochromic fluorescent molecules.14 Although the biological applications of benzopyrylium dyes have been shown recently,15 their environment-sensitive behavior remains unexplored.
image file: c5sc02824h-f1.tif
Fig. 1 Schematic illustrating the rotational freedom and the electron donor–acceptor–donor (D1–A–D2) system in F1.

We then studied the solvatochromic properties of F1 by measuring its absorption and emission spectra in different proportions of water and 1,4-dioxane with different polarities. As shown in Fig. S1 (ESI), all the absorption spectra have maxima at about 598 nm, and there are no significant changes observed in the solutions with different polarities. In contrast, solvent polarity had a dramatic effect on the emission spectra of F1. When the orientation polarizability (Δf) of the solution decreased from 0.32 (99% water) to 0.292 (20% water),16 the maximum emission wavelength of F1 shifted from 646 to 635 nm, concomitant with a gradual increase in fluorescence intensity (Fig. 2a). The fluorescence intensity of F1 at 635 nm increased by a factor of 14.2. The above results reveal that F1 is a polar-sensitive (solvatochromic) fluorescent dye.


image file: c5sc02824h-f2.tif
Fig. 2 (a) Fluorescence spectra of F1 in water/1,4-dioxane solvent mixtures with different fractions of 1,4-dioxane (fd). (b) Fluorescence spectra of F1 in methanol/glycerol solvent mixtures with different fractions of glycerol (fd). F1, 1.0 μM; λex = 580 nm.

Moreover, the multiple electron-donating groups in F1 are linked to the benzopyrylium unit via a single bond. Thus, it features high rotational flexibility and possesses two different twisted intramolecular charge transfer (TICT) channels within the whole molecule (involving twisting of the dimethylaminophenyl and diethylamino groups, respectively, as shown in Fig. 1).17 These intramolecular rotations lead to the nonradiative deactivation of the fluorescent excited state, which might be another cause for the fluorescence quenching of F1 in aqueous solution. To test this assumption, we checked the effect of solvent viscosity on F1 emission. The intramolecular rotation process is reported to be influenced by the viscosity of the medium: the higher the viscosity of the medium, the slower the intramolecular rotation and hence the stronger the F1 emission.18 We then evaluated the viscosity effect on the emission behavior of F1 in methanol/glycerol mixtures with different fractions of glycerol (fg). As shown in Fig. 2b, the emission intensity of F1 is greatly enhanced as the solvent viscosity increases from 0.60 (methanol) to 950 cP (99% glycerol) at room temperature (25 °C),19 which is typically observed for molecular rotors. These experimental results support the fact that F1 is a viscosity-sensitive dye.

Since F1 features a dual dependency of emission intensity on both solvent polarity and viscosity, we thus expect that it would hold great promise in the development of environment-sensitive fluorescent probes compared to the traditional solvatochromic fluorescent dyes. Next, we checked the possible nonspecific interactions of F1 with serum proteins by introducing bovine serum albumin (BSA, 1.0 mg mL−1) to the aqueous solution of F1. As shown in Fig. S2 (ESI), only negligible changes in the emission intensity (2.6-fold increase) of F1 were observed, suggesting there are few nonspecific interactions between F1 and BSA. This is crucial for the development of probes for in vivo imaging, as a high nonspecific background signal is the main reason for their failure. Finally, F1 contains a carboxylic acid group, which enables facile attachment of the recognition moiety. On the basis of the aforementioned results, F1 was selected as the fluorophore to construct the probe.

Additionally, we selected 2-(4-aminophenyl)-1,3,2-dithiarsolane (PAO-EDT) as the recognition unit because its As(III) center can selectively discriminate vicinal dithiols from other forms of thiols through the interchange of 1,2-ethanedithiol (EDT) in cyclic dithiaarsanes with vicinal dithiols in proteins.20 In addition, its 5-membered dithiarsolane ring is a more stable complex compared with the 6- and 7-membered ones.21 In view of the above mentioned results, we rationally designed a red fluorescence turn-on probe FAsH for VDPs in living cells. The detailed synthetic procedures and characterization of FAsH are shown in Scheme 2. Meanwhile, F4 which lacks the 5-membered dithiarsolane ring in its structure was also prepared for comparison purposes.


image file: c5sc02824h-s2.tif
Scheme 2 Synthesis of FAsH and F4. Reagents and conditions: (a) phenylhydrazine, methanol, reflux, 60 min; (b) ethanedithiol, ethanol, reflux, 10 min, 79%; (c) succinic anhydride, toluene, reflux, 3 h, 97%; (d) concentrated H2SO4, 90 °C, 1.5 h, 67%; (e) 4-(N-Boc-amino)piperidine, EDC, HOBt, CH2Cl2, RT, 6 h, 51%. (f) CF3COOH, CH2Cl2, RT, overnight, 73%; (g) PAO-EDT-C, EDC, HOBt, CH2Cl2, RT, 4 h, 54%; (h) succinic anhydride, toluene, reflux, 3 h, 96%; (i) F3, EDC, HOBt, CH2Cl2, RT, 6 h, 51%. Counterions are omitted for clarity. EDC = 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, HOBt = 1-hydroxybenzotriazole.

General spectral properties

With FAsH in hand, we studied its spectral properties. The absorption maximum of FAsH is located at 612 nm in aqueous solution (ε612 nm = 2.68 × 104 M−1 cm−1), which is red-shifted by about 17 nm in comparison with that of F1 (Fig. S3a, ESI). Furthermore, the emission spectra of FAsH were recorded in CH2Cl2 and the results are shown in Fig. S3b (ESI). In agreement with the red-shift in the absorption spectra, the fluorescence spectrum of FAsH displays a 10 nm bathochromic shift when compared to F1. The optical spectra of F4 were almost the same as those of FAsH under identical conditions. Furthermore, it was observed that the fluorescence intensity of FAsH shows no significant difference to that of F4 (Fig. S3b, ESI), indicating that no intramolecular fluorescence quenching was induced by the arsenical moiety, which is apparently different from that seen in bisarsenical dyes.20,22

Optical response

The fluorescence sensing behavior of FAsH toward VDPs was examined. Here, reduced bovine serum albumin (rBSA) was selected as the model protein because it has eight vicinal Cys pairs (its structure is shown in Fig. S4, ESI) after BSA is reduced with tris(2-carboxyethyl)phosphine (TCEP).7b Initially, the kinetic behavior of FAsH toward rBSA was examined in phosphate buffer (20 mM, pH 7.4, containing 1% acetone as cosolvent). Upon introducing rBSA (1.0 equiv.) to the solution of FAsH, a dramatic increase in the fluorescence intensity was observed within 2.5 min, which then reached a plateau as the reaction proceeded, whereas the fluorescence background in the absence of rBSA remained unchanged under identical conditions (Fig. 3). The binding rate of FAsH to rBSA is dramatically accelerated in comparison with the previously reported probe8d which is very important in monitoring the dynamic changes of VDPs in situ. Moreover, upon addition of EDT to the solution of the FAsH–rBSA complex, the fluorescence intensity decreased by 42% within 1 min (Fig. S5, ESI), which proves that the binding of FAsH with rBSA is reversible.20 By contrast, BSA was added to the solution of FAsH and only a negligible fluorescence increase was observed on the time scale of the experiments. These results indicate the high selectivity of FAsH toward vicinal dithiols in proteins.
image file: c5sc02824h-f3.tif
Fig. 3 Time course of the fluorescence intensity of FAsH (1.0 μM) in the presence of rBSA or BSA (both 0.9 μM) in phosphate buffer (20 mM, pH 7.4, containing 1% acetone as cosolvent). λex/λem = 621/651 nm.

The fluorescence response of FAsH toward rBSA was examined by introducing increasing concentrations of rBSA (0–2.4 μM) to the solution of FAsH. As shown in Fig. 4, the free probe gives extremely weak fluorescence in aqueous solution (φf = 0.006, using F1 in CH2Cl2 as a reference).23 However, the addition of an increasing amount of rBSA to the solution of FAsH elicits a gradual increase in the fluorescence intensity and the final enhancement factor is over 70-fold (φf = 0.21). This intensity increase was also accompanied by a hypsochromic shift in the emission spectra from 658 to 651 nm during the titration. The increase in the fluorescence intensity and the hypsochromic shift of the fluorescence emission maxima may be attributed to the binding of FAsH to the hydrophobic domain of rBSA. The fluorescence intensity at 651 nm as a function of rBSA concentration was recorded, and a nearly linear relationship over the range of 0.06–0.9 μM was obtained (Fig. S6, ESI). The detection limit (3δ) for rBSA was calculated to be 0.015 μM. These results demonstrate that FAsH can detect rBSA with high sensitivity. Furthermore, to test the sensing behavior of FAsH toward different VDPs, we examined other reduced forms of proteins (human serum albumin, ovalbumin and lysozome) and found that reduced human serum albumin (rHSA) also induces a dramatic increment in emission intensity, while reduced ovalbumin affords a moderate fluorescence enhancement. In the case of reduced lysozome, a very small increment in emission intensity is observed (Fig. S7, ESI). This is apparently due to different VDPs having different reactivities with FAsH. Thus, FAsH is unsuitable for the quantitative determination of VDP content in complicated biological systems because different VDPs will afford different increments in emission intensity.


image file: c5sc02824h-f4.tif
Fig. 4 Fluorescence spectra (λex = 600 nm) changes for FAsH (1.0 μM) with the addition of increasing concentrations of rBSA (0–2.4 μM) in phosphate buffer (20 mM, pH 7.4, containing 1% acetone as cosolvent) for 5 min. Inset shows the emission intensity at 651 nm as a function of rBSA concentration.

Selectivity studies

To further verify the selectivity of the probe for VDPs, FAsH was treated with a series of biologically relevant species in phosphate buffer (20 mM, pH 7.4). As shown in Fig. 5, only VDPs (rBSA, rHSA and reduced ovalbumin, 1 equiv. of each) induce a significant fluorescence increase, while other amino acids (Ile, Ser, Arg, Met, Thr, Tyr, Ala, Asp, His, Tpc, Pro, Gly, Glc, Trp, Glu, Lys, Leu, Val, Cys, Hcy, GSH, 100 equiv. of each), TCEP, ascorbic acid (100 equiv. of each) and proteins (cytochrome c, myoglobin, lysozyme and BSA, 1 equiv. of each) afford no obvious changes in emission intensity. Furthermore, FAsH can still retain its sensing behavior toward VDPs even in the presence of a large amount of biothiols (GSH, Cys and Hcy) or reductant (ascorbic acid) (Fig. S8, ESI), which further confirms the high selectivity of FAsH towards VDPs over other cellular thiol-containing compounds. The specificity of FAsH toward VDPs was further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). As shown in Fig. 6, a fluorescence band was observed in the lane loaded with rBSA and FAsH, whereas no visible emission was observed in the case of BSA, which contains only one Cys residue (monothiol protein). Furthermore, no fluorescence band was observed when control compound F4 was used for rBSA labeling, which provides strong evidence that the 5-membered dithiarsolane ring in FAsH is responsible for the specific binding of VDPs. By contrast, these protein bands were observed in the gel after silver staining which proves that the fluorescence band is related to the formation of the rBSA–FAsH complex. The excellent selectivity of FAsH toward VDPs can be explained by the fact that the cyclic dithioarsinite complexes (FAsH–rBSA) formed between trivalent arsenicals and vicinal thiols are markedly more stable than the noncyclic products formed from monothiols due to entropic considerations.24
image file: c5sc02824h-f5.tif
Fig. 5 Fluorescence intensity of FAsH (1.0 μM) upon mixing with different species in phosphate buffer (20 mM, pH 7.4, containing 1% acetone as cosolvent) for 5 min. 1, blank; 2, Ile; 3, Ser; 4, Arg; 5, Met; 6, Thr; 7, Tyr; 8, Ala; 9, Asp; 10, His; 11, Tpc; 12, Pro; 13, Gly; 14, Glc; 15, Trp; 16, Glu; 17, Lys; 18, Leu; 19, Val; 20, Cys; 21, GSH; 22, Hcy; 23, TCEP; 24, ascorbic acid (100 equiv. of each); 25, cytochrome c; 26, myoglobin; 27, lysozome; 28, BSA; 29, reduced lysozome; 30, reduced ovalbumin; 31, rBSA; 32, rHSA (1 equiv. of each). λex/λem = 600/651 nm.

image file: c5sc02824h-f6.tif
Fig. 6 The selective binding of FAsH to VDPs was verified by SDS-PAGE. “+”: the compound was present in the detection system; “−”: the compound was absent in the detection system.

Mechanistic study

Some experiments were carried out to gain further insight into the fluorogenic response of FAsH toward VDPs. The absorption spectra of FAsH with the introduction of rBSA were recorded, and it was observed that free FAsH exhibits two absorption bands at 448 and 612 nm. Upon addition of rBSA, a new blue-shifted absorption band centered at 589 nm emerged, and all the absorption bands were increased with increasing rBSA concentration (Fig. S9, ESI). The above spectra variation indicates that FAsH forms H-aggregates in the presence of rBSA.25 Moreover, the aggregates’ formation was evident from the trailing absorption trace of the probe solution in the presence of rBSA due to the light-scattering effects of the dye nanoparticles (Fig. 7 and S9, ESI). In the case of BSA, almost no spectral changes were observed, suggesting that there is almost no interaction between FAsH and BSA. These results indicate that rBSA can induce the aggregation of FAsH selectively. The aggregation was further confirmed using dynamic light scattering (DLS) measurements, and the solution of FAsH (2.0 μM) with rBSA (1.5 μM) shows an average particle size of 180.3 ± 7.3 nm (Fig. S10, ESI). The above experimental results support the rBSA-induced aggregation of FAsH, which can then restrict the intramolecular rotation and thus lead to a great enhancement in the emission intensity.26
image file: c5sc02824h-f7.tif
Fig. 7 Absorption spectra of FAsH (10 μM) in the presence of rBSA or BSA (both 0.3 μM) in phosphate buffer (20 mM, pH 7.4, containing 1% acetone as cosolvent).

Furthermore, to prove the fluorescence enhancement of the sensing process is caused by the hydrophobic pocket of rBSA, guanidine hydrochloride (GdnHCl), a strong protein denaturant, was introduced into the solution of the FAsH–rBSA complex, and a significant decrease in emission intensity was observed (Fig. S11, ESI). This is apparently due to the unfolding of rBSA and the hydrophobic pocket in rBSA is destroyed.27 As a result, the probe gets more exposed to the polar environment, which is undesirable for fluorescence emission. The above results reveal the essential role of the hydrophobic cavities of the protein folding structure in the present sensing system. Collectively, we can conclude that the fluorescence enhancement of the present sensing system is achieved by reducing the charge transfer between the fluorophore and the polar media and restricting the intramolecular rotations via aggregation simultaneously (Scheme 1b).

Application of FAsH in biological systems

In order to show its application in biological samples, we examined the feasibility of FAsH for VDP assays in fetal bovine serum (FBS) samples. The introduction of FAsH to FBS solution induced a slight fluorescence enhancement. However, upon adding dithiothreitol (DTT, 5 mM) to the above mixture, the fluorescence intensity increased progressively (Fig. S12, ESI). As a control, it was observed that DTT alone exhibits no fluorescence increase in FAsH solution. The above experiments prove that FAsH can selectively respond to VDPs in biological samples.

Next, some experiments were performed to evaluate FAsH in live-cell imaging assays using human hepatoma cells (SMMC-7721) as a model cell line. Initially, the cytotoxicity of FAsH was evaluated using a standard MTT assay. Although PAO is quite toxic, the results showed that FAsH has minimal cytotoxicity at concentrations of 2–20 μM (Fig. S13, ESI). This is apparently due to the EDT caging unit, which prevents FAsH from exerting acutely toxic effects.20 Next, SMMC-7721 cells were incubated with FAsH for 20 min in PBS, and a strong fluorescence signal was observed. Furthermore, in a control experiment, the cells were pretreated with 30 μM PAO (a selective VDP binding reagent) to reduce the amount of intracellular free VDPs prior to incubation with FAsH. A pronounced fluorescence quenching was observed (Fig. S14, ESI), which reveals that the above fluorescence emission (Fig. 8) is indeed induced by VDPs. By contrast, F4 was used for cell staining and it affords negligible fluorescence emission under the same conditions (Fig. 8). The semiquantitative calculation of the averaged fluorescence intensity was further conducted. The emission intensity of FAsH-stained cells is about 19-fold higher than that of F4-treated cells (Fig. S15, ESI). The significant difference in emission intensity indicates the selective binding of FAsH to endogenous VDPs inside live cells, and this selective binding is apparently due to the 5-membered dithiarsolane ring. These results demonstrate the capacity of FAsH for in situ imaging of VDPs in living cells.


image file: c5sc02824h-f8.tif
Fig. 8 Confocal fluorescence images of intracellular VDPs in SMMC-7721 cells by FAsH or F4. (a) and (d) The cells were stained with DAPI (1.0 μg mL−1); (b) and (e) the cells were stained with FAsH and F4 (both 5.0 μM), respectively; (c) overlay of (a) and (b); (f) overlay of (d) and (e). Scale bar: 15 μm.

To further investigate the subcellular localization of VDPs, a commercially available mitochondrial tracker (rhodamine 123) was used for a colocalization study with confocal microscopy. As displayed in Fig. 9, the observed fluorescence signal from FAsH extensively overlaps with that of rhodamine 123, implying that FAsH-labeled VDPs are mainly localized to the mitochondria of these live cells. Furthermore, nuclear staining with DAPI indicates that the cells are viable throughout the imaging experiments. The above experiments prove that there is an abundance of VDPs distributed in the mitochondria of SMMC-7721 cells, which is consistent with previous studies.8a,28


image file: c5sc02824h-f9.tif
Fig. 9 FAsH colocalizes to the mitochondria in SMMC-7721 cells. The cells were stained with (a) 2.5 μg mL−1 rhodamine 123, (b) 5.0 μM FAsH, and (c) overlay of (a) and (b) with DAPI (1.0 μg mL−1) stain.

Conclusions

In summary, we have developed a fluorescent light-up probe FAsH for selective detection of VDPs using a unique environment-sensitive flavylium dye F1 as the fluorescent reporter. The probe is almost non-fluorescent in its free form, but exhibits strong fluorescence emission upon specifically binding to VDPs. Therefore, its application no longer requires tedious washing steps during protein labeling, which is favorable for the direct, noninvasive tracing of VDPs in a cellular redox environment. Compared with the widely used solvatochromic fluorescent probes, FAsH possesses the characteristics of two types of environment-sensitive fluorophores (molecule rotors and solvatochromic fluorescent dyes) simultaneously. Thus, it shows a more sensitive light-up fluorescence response toward VDPs. Preliminary imaging experiments reveal that FAsH can serve as a unique probe for the no-wash visualization of endogenous VDPs in living cells. In addition, FAsH has the advantage of rapid binding kinetics, a high signal-to-noise ratio, red emission and will be an attractive tool for the in situ investigation of the essential role of VDPs in intracellular redox homeostasis and the exploration of its diverse pathophysiology.

Acknowledgements

This research was supported by the National Natural Science Foundation of China (no. 21475105, 21275117, 21375105), the Science & Technology Department (no. 2012JM2004) and the Education Department (no. 12JK0581) of Shaanxi Province of China.

Notes and references

  1. C. Gitler, B. Zarmi and E. Kalef, Anal. Biochem., 1997, 252, 48–55 CrossRef CAS PubMed .
  2. C. C. Winterbourn, Nat. Chem. Biol., 2008, 4, 278–286 CrossRef CAS PubMed .
  3. M. Valko, C. J. Rhodes, J. Moncol, M. Izakovic and M. Mazur, Chem.-Biol. Interact., 2006, 160, 1–40 CrossRef CAS PubMed .
  4. E. Yoshihara, S. Masaki, Y. Matsuo, Z. Chen, H. Tian and J. Yodoi, Front. Immunol., 2013, 4, 514 Search PubMed .
  5. M. L. Alexandrova and P. G. Bochev, Biol. Med., 2005, 39, 297–316 CAS .
  6. G. Bánhegyi, J. Mandl and M. Csala, J. Neurochem., 2008, 107, 20–34 CrossRef PubMed .
  7. (a) Y. Chen, C. M. Clouthier, K. Tsao, M. Strmiskova, H. Lachance and J. W. Keillor, Angew. Chem., Int. Ed., 2014, 53, 13785–13788 CrossRef CAS PubMed ; (b) X. Pan, Z. Liang, J. Li, S. Wang, F. Kong, K. Xu and B. Tang, Chem.–Eur. J., 2015, 21, 2117–2122 CrossRef CAS PubMed ; (c) S. Girouard, M.-H. Houle, A. Grandbois, J. W. Keillor and S. W. Michnick, J. Am. Chem. Soc., 2005, 127, 559–566 CrossRef CAS PubMed .
  8. (a) C. Huang, Q. Yin, W. Zhu, Y. Yang, X. Wang, X. Qian and Y. Xu, Angew. Chem., Int. Ed., 2011, 50, 7551–7556 CrossRef CAS PubMed ; (b) C. Huang, Q. Yin, J. Meng, W. Zhu, Y. Yang, X. Qian and Y. Xu, Chem.–Eur. J., 2013, 19, 7739–7747 CrossRef CAS PubMed ; (c) N. Fu, D. Su, J. R. Cort, B. Chen, Y. Xiong, W.-J. Qian, A. E. Konopka, D. J. Bigelow and T. C. Squier, J. Am. Chem. Soc., 2013, 135, 3567–3575 CrossRef CAS PubMed ; (d) C. Huang, T. Jia, M. Tang, Q. Yin, W. Zhu, C. Zhang, Y. Yang, N. Jia, Y. Xu and X. Qian, J. Am. Chem. Soc., 2014, 136, 14237–14244 CrossRef CAS PubMed .
  9. (a) E. C. Webb and R. van Heyningen, Biochem. J., 1947, 41, 74–78 CrossRef CAS ; (b) V. P. Whittaker, Biochem. J., 1947, 41, 56–62 CrossRef CAS .
  10. (a) P. M. Loiseau, P. Lubert and J. G. Wolf, Antimicrob. Agents Chemother., 2000, 44, 2954–2961 CrossRef CAS ; (b) Y. Liu, D. Duan, J. Yao, B. Zhang, S. Peng, H. Ma, Y. Song and J. Fang, J. Med. Chem., 2014, 57, 5203–5211 CrossRef CAS PubMed .
  11. (a) Y.-D. Zhuang, P.-Y. Chiang, C.-W. Wang and K.-T. Tan, Angew. Chem., Int. Ed., 2013, 52, 8124–8128 CrossRef CAS PubMed ; (b) Q. Sun, J. Qian, H. Tian, L. Duan and W. Zhang, Chem. Commun., 2014, 50, 8518–8521 RSC .
  12. (a) G. S. Loving, M. Sainlos and B. Imperiali, Trends Biotechnol., 2009, 28, 73–83 CrossRef PubMed ; (b) A. S. Klymchenko and Y. Mely, Prog. Mol. Biol. Transl. Sci., 2013, 113, 35–58 CAS ; (c) E. Yamaguchi, C. Wang, A. Fukazawa, M. Taki, Y. Sato, T. Sasaki, M. Ueda, N. Sasaki, T. Higashiyama and S. Yamaguchi, Angew. Chem., Int. Ed., 2015, 54, 4539–4543 CrossRef CAS PubMed .
  13. L. Yuan, W. Lin, K. Zheng, L. He and W. Huang, Chem. Soc. Rev., 2013, 42, 622–661 RSC .
  14. (a) J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Springer, 3rd edn, 2006 Search PubMed ; (b) H. Wang, J. Liu, A. Han, N. Xiao, Z. Xue, G. Wang, J. Long, D. Kong, B. Liu, Z. Yang and D. Ding, ACS Nano, 2014, 8, 1475–1484 CrossRef CAS PubMed .
  15. (a) H. Chen, W. Lin, W. Jiang, B. Dong, H. Cui and Y. Tang, Chem. Commun., 2015, 51, 6968–6971 RSC ; (b) L. Yuan, F. Jin, Z. Zeng, C. Liu, S. Luo and J. Wu, Chem. Sci., 2015, 6, 2360–2365 RSC ; (c) H. Lv, X.-F. Yang, Y. Zhong, Y. Guo, Z. Li and H. Li, Anal. Chem., 2014, 86, 1800–1807 CrossRef CAS PubMed ; (d) Y. Chen, X. Wang, X.-F. Yang, Y. Zhong, Z. Li and H. Li, Sens. Actuators, B, 2015, 206, 268–275 CrossRef CAS PubMed ; (e) J. Liu, Y.-Q. Sun, J. Zhang, T. Yang, J. Cao, L. Zhang and W. Guo, Chem.–Eur. J., 2013, 19, 4717–4722 CrossRef CAS PubMed ; (f) W. Chen, Q. Fang, D. Yang, H. Zhang, X. Song and J. Foley, Anal. Chem., 2015, 87, 606–616 Search PubMed .
  16. N. Jiang, J. Fan, F. Xu, X. Peng, H. Mu, J. Wang and X. Xiong, Angew. Chem., Int. Ed., 2015, 54, 2510–2514 CrossRef CAS PubMed .
  17. W. Rettig and W. Majenz, J. Photochem. Photobiol., A, 1992, 62, 415–427 CrossRef CAS .
  18. (a) M. A. Haidekker and E. A. Theodorakis, Org. Biomol. Chem., 2007, 5, 1669–1678 RSC ; (b) M. K. Kuimova, G. Yahioglu, J. A. Levitt and K. Suhling, J. Am. Chem. Soc., 2008, 130, 6672–6673 CrossRef CAS PubMed .
  19. Z. Yang, Y. He, J. H. Lee, W.-S. Chae, W. X. Ren, J. H. Lee, C. Kang and J. S. Kim, Chem. Commun., 2014, 50, 11672–11675 RSC .
  20. B. A. Griffn, S. R. Adams and R. Y. Tsien, Science, 1998, 281, 269–272 CrossRef .
  21. W. L. Zahler and W. W. Clelland, J. Biol. Chem., 1968, 243, 716–719 CAS .
  22. R. A. Scheck and A. Schepartz, Acc. Chem. Res., 2011, 44, 654–665 CrossRef CAS PubMed .
  23. P. Czerney, G. Graneβ, E. Birckner, F. Vollmer and W. Rettig, J. Photochem. Photobiol., A, 1995, 89, 31–36 CrossRef CAS .
  24. (a) L. A. Stocken and R. H. Thompson, Biochem. J., 1946, 40, 535–548 CrossRef CAS ; (b) A. M. Spuches, H. G. Kruszyna, A. M. Rich and D. E. Wilcox, Inorg. Chem., 2005, 44, 2964–2972 CrossRef CAS PubMed .
  25. (a) M. Ogawa, N. Kosaka, P. L. Choyke and H. Kobayashi, ACS Chem. Biol., 2009, 4, 535–546 CrossRef CAS PubMed ; (b) N. C. Maiti, S. Mazumdar and N. Periasamy, J. Phys. Chem. B, 1998, 102, 1528–1538 CrossRef CAS .
  26. (a) Y. Hong, J. W. Y. Lam and B. Z. Tang, Chem. Commun., 2009, 4332–4353 RSC ; (b) Y. Hong, J. W. Y. Lam and B. Z. Tang, Chem. Soc. Rev., 2011, 40, 5361–5388 RSC .
  27. Y. Hong, C. Feng, Y. Yu, J. Liu, J. W. Y. Lam, K. Q. Luo and B. Z. Tang, Anal. Chem., 2010, 82, 7035–7043 CrossRef CAS PubMed .
  28. R. Requejo, E. T. Chouchani, A. M. James, T. A. Prime, K. S. Lilley, I. M. Fearnley and M. P. Murphy, Arch. Biochem. Biophys., 2010, 504, 228–235 CrossRef CAS PubMed .

Footnote

Electronic supplementary information (ESI) available: Experimental procedures, characterization data, and additional spectra. See DOI: 10.1039/c5sc02824h

This journal is © The Royal Society of Chemistry 2016
Click here to see how this site uses Cookies. View our privacy policy here.