Lan-Gui
Xie
and
Darren J.
Dixon
*
Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK. E-mail: darren.dixon@chem.ox.ac.uk
First published on 11th September 2017
A new iridium catalyzed reductive coupling reaction of Grignard reagents and tertiary amides affording functionalised tertiary amine products via an efficient and technically-simple one-pot, two-stage experimental protocol, is reported. The reaction – which can be carried out on gram-scale using as little as 1 mol% Vaska's complex [IrCl(CO)(PPh3)2] and TMDS as the terminal reductant for the initial reductive activation step – tolerates a broad range of tertiary amides from (hetero)aromatic to aliphatic (branched, unbranched and formyl) and a wide variety of alkyl (linear, branched), vinyl, alkynyl and (hetero)aryl Grignard reagents. The new methodology has been applied directly to bioactive molecule synthesis and the high chemoselectivity of the reductive coupling of amide has been exploited in late stage functionalization of drug molecules. This reductive functionalisation of tertiary amides provides a new and practical solution to tertiary amine synthesis.
Reductive functionalisation of amide functionality to generate carbon–carbon bonds alpha to nitrogen has also been demonstrated as a viable approach for amine synthesis.6–8 In this context, approaches using stoichiometric amounts of strongly electrophilic reagents, such as Tf2O and POCl3, for amide pre-activation prior to reductive functionalisation, have been described9 (Scheme 1A). Weinreb amides have also been demonstrated as alternative substrates for reductive pre-activation, using stoichiometric DIBAL-H or Schwartz's reagent (Cp2ZrHCl), affording alkoxy amine products after, for example, allylation and cyanation reactions in the presence of Lewis acids for reactive iminium ion formation10 (Scheme 1B). Although the above stoichiometric approaches are demonstrably successful, catalysed variants of these reductive functionalization processes offer a number of potential benefits, especially in terms of chemoselectivity and functional group tolerance. For example, in presence of Vaska's complex and (Me2HSi)2O (TMDS), Chida/Sato11 demonstrated the formation of hemiaminal intermediates from Weinreb amide substrates, which with the assistance of stoichiometric BF3·Et2O, undergo substitution by preformed nucleophiles such as silyl ketene acetals and allyltin reagents. Our group, and others, have been exploring amine synthesis through exploitation of enamine and iminium ion intermediates formed by catalytic partial reduction of tertiary amides.12 In this respect, we have reported the iridium catalysed reductive intramolecular nitro-Mannich reaction of lactams for the synthesis of bicyclic nitroamines, and the broad-scope iridium catalysed reductive Strecker reaction of tertiary amides for the efficient synthesis of α-amino nitrile products.
In a continuation of our program, and with the ultimate aim of expanding the range of amine products accessible using the two stage reductive activation approach, we considered a reductive coupling of tertiary amides and Grignard reagents (Scheme 1C). Grignard reagents are widely available commercial organometallic reagents and are simply and reliably prepared in the laboratory. Similarly, tertiary amides are commonly found in biologically active natural products and drug compounds, and are widespread throughout pharmaceutical and agrochemical companies. When combined with the ease at which they can be prepared through standard amide coupling reactions,13 a mild reductive coupling protocol that could efficiently and chemoselectively target this functional group to generate tertiary amine products from Grignard reagents would likely find numerous applications in preparative scale synthesis, library generation, late-stage functionalisation, and total synthesis alike. Herein we wish to report our findings.
Entry | Cat. loading (mol%) | Solvent | Concentration (M) | Yieldb (%) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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a Reactions were performed on 0.3 mmol of amide. b NMR yields with 3,5-dibromoanisole as internal standard. c Isolated yield. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 | 1 | Tetrahydrofuran | 0.05 | 54 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2 | 1 | Toluene | 0.05 | 64 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3 | 1 | CH2Cl2 | 0.05 | 82 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4 | 3.3 | CH2Cl2 | 0.05 | 85 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5 | 0.5 | CH2Cl2 | 0.05 | 53 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6 | 1 | CH2Cl2 | 0.1 | 95(89)c | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7 | 1 | CH2Cl2 | 0.025 | 35 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8 | 1 | CH2Cl2 | 0.5 | 80 |
With optimised conditions in hand the scope of the reductive benzylation reaction with respect to the tertiary amide was assessed (Scheme 2). Benzoylamides derived from cyclic amines pyrrolidine, azepine, azocane, morpholine and 6,7-dimethoxytetrahydroisoquinoline respectively, all furnished the target tertiary amines (2–6) in good to excellent yields (70–90%). Methylbenzylbenzoylamide was also a good substrate yielding benzylamine (7) in excellent yield and thus expanding the potential application of the protocol to secondary amine synthesis via subsequent hydrogenolysis. The Weinreb amide of benzoic acid was an excellent substrate and afforded α-benzylated methoxyamine product 8 in 87% yield. Methylaniline derived benzoyl amide was also amenable to reductive benzylation, and furnished a substituted aniline product 9. The acceptable yield of 57% in this case required the use of an increased loading of Vaska's complex (5 mol%) in the initial reductive activation step to overcome diminished substrate reactivity. Great tolerance with respect to the arene moiety of the dimethylamide was witnessed under the optimised reaction conditions. Thus electron deficient, electron-rich and halogen-substituted benzoic acid derivatives gave the respective tertiary amine products (14–18) in yields ranging from 84 to 97%. Similarly a 2-furanyl substrate gave the desired amine product (19) in 94% yield. Aliphatic dimethyl amides, derived from n-decanoic acid, cyclohexane carboxylic acid and pivalic acid were all good substrates for the reductive alkylation protocol and yielded tertiary amine products 10–12 in respectable yields. N-Formyl piperidine was also an excellent substrate and gave product 13 in 82% yield. The ability to dialkylaminomethylate organomagnesium reagents using tertiary formamides should find many synthetic applications within target synthesis, methodology development and drug discovery programs. Unfortunately, under the current reaction conditions, reductive coupling of lactams with Grignard reagents were not successful.14
Despite the use of a relatively reactive organometallic coupling partner (BnMgCl) the functional group tolerance of the reductive functionalisation reaction was found to be high. Nitrile, aryl ketone, pinacol boronate ester, tert-butyl carbamate and ester functionality all survived when the amount of Grignard reagent in the second stage was lowered to 1.2 equivalents and the reaction temperature kept below 0 °C. The ability to tolerate halide functionality and pinacol boronate esters establishes this transformation as fully compatible with other cross-coupling reactions, indicating its potential for the synthesis of amine derived building blocks.
To demonstrate the synthetic utility of this newly developed reductive carbon–carbon bond forming methodology, we selected several amine-containing drug molecules as targets. NMDA receptor antagonists, diphenidine (25), methoxphenidine (26) and fluorolintane (27), which are generally used as dissociative anesthetics,15 were obtained directly and efficiently by submitting the corresponding and readily prepared parent amides to the standard protocol. Likewise ephenidine (28), a secondary amine drug molecule,16 was synthesised by partial hydrogenolysis of the initially generated tertiary amine from its benzylethyl benzoylamide precursor, in 67% yield over the two steps. Furthermore, piperazine-derived opioid analgesic drug MT-45(29)17 was prepared in near quantitative yield.
The scope with respect to the Grignard reagent was also found to be broad. As depicted in Scheme 3, methyl, linear-alkyl and branched-alkyl magnesium reagents were compatible with this protocol and yielded amine products (30–32) in good to excellent yields. (Trimethylsilyl)methylmagnesium chloride was also an excellent reagent for new carbon–carbon bond formation under the standard conditions. Importantly, a reductive methylation of N,N-dimethyl-p-toluamide was carried out on gram scale following the standard protocol and afforded methylated product 34 in 85% yield. Reductive vinylation using vinylmagnesium bromide and reductive alkynylation using hexynyl magnesium bromide yielded allylamine 35 and propargylamine 36 in 74% and 86% yield, respectively. Phenyl magnesium bromide was also found to be a competent nucleophilic coupling partner and afforded benzylic tertiary amine product 37 in 66% respectively. Reductive phenylation [or (hetero)arylation] of amides will likely find many applications within the pharmaceutical sector and here we have exemplified it through the synthesis of the antiemetic piperazine-containing drug cinnarizine (38).18 Using the two-step protocol 38 was successfully synthesised in 67% yield using phenylmagnesium bromide in the carbon–carbon bond-forming step.
Encouraged by the high reactivity of the silylated hemiaminal intermediates towards organomagnesium reagents, we then chose to apply Knochel-type Grignard reagents to the new reductive carbon–carbon bond forming methodology. The diversity of organomagnesium reagents accessible by the Knochel procedure is impressive and would therefore boost the nature and type of amine products accessible using this approach.19 To this end, para-trifluoromethylphenyl organomagnesium reagent, prepared by magnesium/iodide exchange with isopropylmagnesium bromide, was investigated in the reductive arylation of the pyrrolidine amide of benzoic acid and indeed was found to be compatible with our protocol, yielding 39 in 65% yield. Similarly a range of aryl and pyrimidine-based heteroaryl Knochel-type Grignard reagents were successfully generated, then reacted with N-formyl piperidine following our standard protocol, furnishing homologated amine products (40–42) in satisfactory yields. Furthermore, organomagnesium reagents derived from α-D-glucofuranose and cholesterol derivatives using the Knochel procedure were also compatible.
The synthetic utility of this chemistry was further demonstrated through the late stage reductive functionalisation of drug molecules. Thus, piperine20 (an extract of black pepper used as traditional medicine and insecticide) was successfully modified by benzylation (45, 93%) and butenylation (46, 83%). Also, the psychoactive drug fipexide21 underwent a reductive coupling reaction with benzylmagnesium chloride affording 47 in 73% yield. While by magnesium/proton exchange of the acetylenic proton of pargyline22 (an irreversible selective monoamine oxidase MAO-B inhibitor) and then homologation with N-formyl piperidine, amine derivative 48 was obtained in 54% yield.
Footnote |
† Electronic supplementary information (ESI) available: Experimental procedures, spectroscopic data, copies of 1H and 13C NMR spectra and crystallographic data. CCDC 1552384. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7sc03613b |
This journal is © The Royal Society of Chemistry 2017 |