Issue 61, 2020
From the journal:

Chemical Communications

A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC†

Stefanie Brands, ORCID logo a   Hannah U. C. Brass, ORCID logo b   Andreas S. Klein, ORCID logo b   Jörg Pietruszka, ORCID logo bc   Anna Joëlle Ruff ORCID logo a  and  Ulrich Schwaneberg ORCID logo *ad  

* Corresponding authors

a Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany
E-mail: u.schwaneberg@biotec.rwth-aachen.de

b Institute of Bioorganic Chemistry, Heinrich Heine University Düsseldorf located at Forschungszentrum Jülich, Stetternicher Forst, Building 15.8, 52426 Jülich, Germany

c Institute of Bio- and Geosciences (IBG-1: Biotechnology), Forschungszentrum Jülich, 52426 Jülich, Germany

d DWI-Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52056 Aachen, Germany

Abstract

A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.

Graphical abstract: A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

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Article information

DOI
https://doi.org/10.1039/D0CC02181D
Article type
Communication
Submitted
24 Mar 2020
Accepted
17 Jun 2020
First published
18 Jun 2020

Chem. Commun., 2020,56, 8631-8634

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A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

S. Brands, H. U. C. Brass, A. S. Klein, J. Pietruszka, A. J. Ruff and U. Schwaneberg, Chem. Commun., 2020, 56, 8631 DOI: 10.1039/D0CC02181D

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