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Synthesis, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities, and molecular docking studies of a novel compound based on combination of flurbiprofen and isoniazide

Amina Asgharab, Muhammad Yousufc, Ghulam Fareedd, Rabia Nazire, Abida Hassanf, Aneela Maalikg, Tayyaba Noorh, Naseem Iqbali and Lubna Rasheed*a
aDepartment of Chemistry, Division of Science and Technology, University of Education, Township, Lahore, Pakistan. E-mail: lubnarasheed@ue.edu.pk
bDepartment of Chemistry, University of Oxford, Oxford, UK
cDepartment of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea
dPharmaceutical Research Centre, Pakistan Council of Scientific and Industrial Research, Karachi, Pakistan
ePakistan Council of Scientific and Industrial Research, Lahore, Pakistan
fHigher Education Commission, Punjab, Pakistan
gCOMSATS University Islamabad, Islamabad 45550, Pakistan
hSchool of Chemical and Materials Engineering (SCME), National University of Science and Technology (NUST), Islamabad 44000, Pakistan
iUS Pakistan Centre of Advanced Studies in Energy (USPCAS-E), National University of Science and Technology (NUST), Islamabad 44000, Pakistan

Received 12th March 2020 , Accepted 4th May 2020

First published on 20th May 2020


Abstract

Synthesis of a compound with balanced bioactivities against a specific target is always a challenging task. In this study, a novel compound (1) has been synthesized by combination of flurbiprofen and isoniazide and shows ∼2.5 times enhanced acetylcholinesterase (AChE) inhibition activity and ∼1.7 times improved butyrylcholinesterase (BuChE) inhibition activity compared to flurbiprofen and a standard drug (i.e. physostigmine). A comparative AutoDock study has been performed, based on the optimized structure, by the DFT/B3LYP method, which confirmed that compound (1) is more active against AChE and BuChE, with calculated binding energies of −12.9 kcal mol−1 and −9.8 kcal mol−1 respectively as compared to flurbiprofen and an eserine (physostigmine) standard for which the binding energy was calculated to be −10.1 kcal mol−1 and −8.9 kcal mol−1, respectively. A mixed mode of inhibition of AChE and BuChE with compound 1 was confirmed by Lineweaver–Burk plots. AChE and BuChE inhibition activity alongside docking results suggests that compound (1) could be used for treatment of Alzheimer's disease. Moreover, compound (1) also exhibit better α-chymotrypsin activity compared to flurbiprofen. Furthermore, in vitro and in vivo analysis confirmed that compound (1) exhibit more activity and less toxicity than the parent compounds.


Introduction

The history of non-steroidal anti-inflammatory drugs (NSAIDs) dates back thousands of years but the mechanism of NSAID therapies was first fully understood by John Vane in 1971.1–3 Nowadays, NSAIDs (naproxen, indomethacin, aspirin, ibuprofen, flurbiprofen and diclofenac) have been recommended for diseases such as pain, short term fever and inflammation.4–7 Scientists have worked hard to understand the kinetics and structure–activity relationships of these drugs.8,9 In this regard, computer-aided drug design (CADD) is one of the most powerful tools.10–13 It enables us to search and understand the interactions of ligands with potential protein targets.14 Moreover, structure–activity relationships can be established in a better way by comparing the experimental results with theoretical studies. Therefore, CADD has an intrinsic benefit of understanding the phenomenon at the molecular level and providing correct assignments.15

A patient suffering from Alzheimer's disease (AD) experience a progressive and irreversible disorder of brain which slowly destroy thinking and memory skills at large.16 AD is currently ranked as the sixth leading cause of death in United States and there are almost 50 million people around the world suffering from this disease.16–18 It has been observed that change in acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity in cerebral cortex and hippocampus contribute to the disease progression.17–19 Increased or unchanged BuChE activity and decreased AChE activity are commonly observed in certain brain regions of patients suffering from AD.20–22 Human AChE active site, a long gorge with overall length of approximately 20 Å, mainly consisting of catalytic active site (CAS) at bottom of gorge while peripheral anionic site (PAS) is situated near the entrance of gorge. These two are linked by a narrow groove. CAS forms the catalytic triad, Ser200, Glu327 and His440, and is responsible for hydrolysis of AChE inside the triad.23,24 PAS consists of several aromatic residues, including Tyr70, Tyr121, and Trp279. Compounds that can interact with both CAS and PAS are believed to exert multiple therapeutic effects.25 The shape and arrangement of the active site of BuChE is similar to that of AChE; however, the volume of the catalytic site in BuChE is much larger than that of AChE.26 Researchers from all over the world are trying to explore new and novel strategies to develop effective methods for treatment of AD and other diseases as well, but it is a challenging task to obtain a specific compound with balanced activities against the specific targets most importantly retaining the drug-like properties.27 Synthesis of novel drug by using existing NSAIDs is an area which is largely unexplored and it has been observed that NSAIDs can be tuned for better lipophilicity, reduced toxicity and better bio-availability.28 Therefore a clever design and synthesis of compound having aforementioned properties is need of the day in order to grasp and improve the pharmacological benefits of NSAIDs.29

Keeping in view of the facts about AD and limitations of existing drugs, a novel compound based on combination of flurbiprofen and isoniazide, N′-(2-(2-fluoro-[1,1′-biphenyl]-4-yl)propanoyl) isonicotinohydrazide (1), has been synthesized. The synthesized compound (1) was subjected to in vitro analysis which shows ∼2.5 times and ∼1.7 times enhanced AChE and BuChE inhibition activity respectively compared to flurbiprofen and standard physostigmine. These experimental results has been supported by comparative AutoDock study of compound (1) with AChE and BuChE which also confirmed that compound (1) is more active against AChE and BuChE with binding energy of −12.9 kcal mol−1 and −9.8 kcal mol−1 respectively as compared to flurbiprofen for which binding energy was calculated to be −8.2 kcal mol−1. Compound (1) showed improved α-chymotrypsin activity as well. Lineweaver–burk plot suggests mixed mode of inhibition of AChE and BuChE with compound 1. Experiments suggests that the compound 1 is slightly more lipophilic compared to flurbiprofen. AChE and BuChE inhibition activities aided with docking results suggested that compound (1) could be used for Alzheimer's disease. To the best of our knowledge, no report has been published yet which describes synthesis, experimental procedures, molecular docking and in vitro studies of the compound (1). Furthermore, the present data as theoretically and experimentally can be helpful for further studies of compounds/derivatives of flurbiprofen.

Results and discussion, experimental

Synthesis

Synthesis of the compound (1) was done by preparing acid chloride of flurbiprofen using oxalyl chloride which was then allowed to react with isoniazide. Scheme 1 (detail in ESI).
image file: d0ra02339f-s1.tif
Scheme 1 Synthesis of novel compound (1) based on combination of flurbiprofen and isoniazide.

Biological activities

Anti-inflammatory activities. Percent inhibitions of edema by the flurbiprofen, isoniazide and the compound (1) at dose rate of 50 mg kg−1 were found to be 95.81, 28.51 and 75.21 respectively (Table 1). Although anti-inflammatory activity of the compound is slightly less than flurbiprofen but IC50 value is far greater than the parent drug which can be advantageous in treatment. A control experiment had also been performed using flurbiprofen and isoniazide at half dose. Again the compound 1 showed more activity compared to flurbiprofen and isoniazide at half dose. The existence of polar groups i.e. fluoro and amide alongwith hydrogen bond acceptor (pyridyl) side of compound 1 might be the main cause of better anti-inflammatory activity.
Table 1 Anti-inflammatory assay of compound (1) and parent drugs
Anti-inflammatory drug Inhibition (%) IC50 (μmol)
Isoniazide 28.51 ± 0.12
Flurbiprofen 95.81 ± 0.16 50.51 ± 0.14
Flurbiprofen[thin space (1/6-em)]:[thin space (1/6-em)]isoniazide (50[thin space (1/6-em)]:[thin space (1/6-em)]50) 62.31 ± 0.11
Compound 1 75.21 ± 0.16 352.2 ± 0.27


Enzyme inhibition activities.
In vitro acetylcholinesterase (AChE) inhibition assays. Acetylcholine (ACh) has been proved to employ an anti-inflammatory property with action that involves down-modulating of pro-inflammatory cytokines.30 Therefore, AChE is responsible for hydrolysis of acetylcholine and is being modulated in inflammation. Results showed that AChE inhibitory activity of the compound (1) was considerably greater (∼2.5 times) than that of flurbiprofen (Table 2). Physostigmine (eserine) has been used as a standard compound in order to compare the activity with that of flurbiprofen and compound 1. These results suggest that compound (1) offer selective and far improved inhibition of AChE as compared with flurbiprofen and standard (physostigmine). Based on experimental results and further confirmed by molecular studies it has been suggested that the synthesized compound (1) can be used for treatment of AD.
Table 2 AChE assay of compound (1) compared with parent drugs
Drugs used as standard Inhibition (%) IC50 (μmol)
Isoniazide 15.19 ± 0.04 1.11 ± 0.17
Flurbiprofen 31.43 ± 0.05 50.51 ± 0.14
Flurbiprofen[thin space (1/6-em)]:[thin space (1/6-em)]isoniazide (50[thin space (1/6-em)]:[thin space (1/6-em)]50) 22.31 ± 0.07
Physostigmine (eserine) 45.35 ± 0.06
Compound 1 78.92 ± 0.24 112.11 ± 0.14



In vitro butyrylcholinesterase (BuChE) inhibition assays. Compound 1 was also checked for its inhibitory activity against BuChE as well. Here again physostigmine (eserine) has also been used as a standard. The results showed that the BuChE inhibitory activity of compound 1 was almost two times higher as compared to flurbiprofen (Table 3) and standard drug. These results suggest that compound (1) also offer selective and improved inhibition of BuChE compared with flurbiprofen.
Table 3 BuChE assay of compound (1) compared with parent drugs
Drugs used as standard Inhibition (%) IC50 (μmol)
Isoniazide 15.51 ± 0.15 20.25 ± 0.18
Flurbiprofen 36.17 ± 0.24 <600
Flurbiprofen[thin space (1/6-em)]:[thin space (1/6-em)]isoniazide (50[thin space (1/6-em)]:[thin space (1/6-em)]50) 23.41 ± 0.05
Physostigmine (eserine) 41.42 ± 0.04
Compound 1 61.25 ± 0.85 238.51 ± 0.11


Structure–activity relationship can be inferred by comparison of the experimental data with molecular docking studies. According to the docking simulation it is clear that compound (1) is able to fit well in active site of acetylcholinesterase and butyrylcholinesterase and interact with important amino acid residues. The enhanced inhibition activity for compound (1) might be due to the presence of flouro group at ortho position of the extended phenyl ring and pyridyl group on other side of molecule. Compound (1) is able to form potential π–π interaction with residue Tyr332 and hydrophobic interactions with other residues within the esteratic pocket of the active site (Fig. 2a). Fluoro and pyridyl groups on sides aided by amide group in the middle tend to stabilize the ground state orientation by engaging in hydrophobic and hydrophilic interactions of amino acids (Fig. 4).

To further investigate the binding manner compound 1 was subjected to kinetic studies with AChE and BuChE, respectively. Lineweaver–Burk reciprocal plots were applied as described previously to elucidate the kinetic properties and inhibitory modes of the compounds.26 Generally, Lineweaver–Burk plots can be described by reciprocal rates versus reciprocal substrate concentrations for different inhibitor concentrations resulting from the substrate–velocity curves for ChEs. The detailed values of Km and Vmax of compound against AChE and BuChE at different concentrations are listed in Table 4. From the Fig. 1a its evident that both slopes (decreased Vm), and the intercepts (higher K) varied with increasing concentration (0.2, 0.4, 0.6, and 1.0 mM). This behaviour suggests mixed inhibition of AChE by compound 1. The substrate–velocity curve (Fig. 1b) showed that compound 1 reduced the enzymatic velocity of the AChE-substrate catalytic reaction in a dose-dependent manner. From Fig. 2 it is proved that compound 1 also exhibited mixed inhibition of BuChE and a dose-dependent decrease of the enzymatic velocity of the BuChE-substrate catalytic reaction. These results indicated that the compound 1 may simultaneously bind to CAS and PAS when interacting with the targets.

Table 4 The Vmax and Km values of compound 1 in kinetic studies with AChE and BuChE
Concentration (μM) Vmax (μM min−1) Km (μM) R2
Against AChE
0 3.86 ± 0.61 354.37 ± 10.34 0.99
0.2 3.69 ± 0.73 429.06 ± 13.26 0.99
0.4 2.52 ± 0.50 317.93 ± 11.95 0.99
0.6 1.70 ± 0.21 254.53 ± 7.32 0.99
1 1.27 ± 0.14 260.56 ± 8.22 0.99
[thin space (1/6-em)]
Against BuChE
0 0.46 ± 0.05 299.69 ± 9.21 0.99
0.2 0.39 ± 0.03 364.54 ± 14.54 0.99
0.4 0.32 ± 0.02 425.17 ± 16.87 0.99
0.6 0.25 ± 0.02 474.13 ± 18.15 0.99
1 0.17 ± 0.02 393.30 ± 17.92 0.99



image file: d0ra02339f-f1.tif
Fig. 1 (a) Lineweaver–burk plot of compound 1 with AChE. (b) Enzyme inhibition activity of compound 1 with AChE.

image file: d0ra02339f-f2.tif
Fig. 2 (a) Lineweaver–burk plot of compound 1 with BuChE. (b) Enzyme inhibition activity of compound 1 with BuChE.

In vitro α-chymotrypsin activity of compound (1) and parent drugs. Chymotrypsin is a digestive enzyme component of pancreatic juice acting and performs proteolysis.31 Chymotrypsin preferentially cleaves peptide amide bonds by hydrolysis reaction and helps in digestion. Generally this enzyme is activated in the presence of trypsin. Compound (1) showed enhanced α-chymotrypsin activity compared with parent drugs (Table 5).
Table 5 α-Chymotrypsin activity of compound (1) compared with parent drugs
Parent drug Concentration of solutions (mM) Inhibition (%)
Isoniazide 0.5 5.13 ± 0.12
Flurbiprofen 0.5 27.05 ± 0.16
Flurbiprofen[thin space (1/6-em)]:[thin space (1/6-em)]isoniazide (50[thin space (1/6-em)]:[thin space (1/6-em)]50) 0.5 16.38 ± 0.12
Compound 1 0.5 33.01 ± 0.15


Toxicity study

The LD50 (oral, rats) values were found to be isoniazide: 645 mg kg−1, flurbiprofen: 115 mg kg−1 and compound (1): 3431 mg kg−1. Toxicity results proved that the compound (1) is much safer to use than the parent analogues.

Lipophilicity studies

In context of drug discovery one of the most important parameter, despite the fact that the drug should show a significant activity against the potential target, is identification of drugs which are more likely to be well absorbed and distributed in human body. It suggests that a drug must be lipophilic enough to penetrate the lipid cores of membranes, but not so lipophilic that they get stuck there.32 Lipophilicity of the compound (1) was calculated by following the recently published method.33 log[thin space (1/6-em)]P of compound (1) was calculated to be 3.94 which is slightly higher than flurbiprofen (log[thin space (1/6-em)]P 3.82). Flurbiprofen possess carboxylic moiety, therefore it is less lipophilic than compound (1) that contains amide linkage.

Molecular docking studies

The study was designed for compound (1) against acetylcholinesterase and butyrylcholinesterase enzymes with the following communications; Intel(R) core i7 @ 3.50 GHz system having 8 GB RAM with windows 7 operating platform. Protein–ligand docking was carried out using Autodock Vina software.34 X-ray crystal structures of human BChE (Pdb: 1P0P with 2.30 Å resolution) from the RCSB protein data bank35 was selected as the target protein based on suitable resolution and co-crystallized ligands, i.e. AChE and BuChE. The energy of ligands was minimized using MMFF94x force field. 3D and 2D interactions diagrams were generated through BIOVIA Discovery Studio visualizer V17.2.36 Before proceeding for docking studies structure of compound (1) was optimized with the help of density functional theory (DFT)/B3LYP37–42 method with 6-311G(d, p) as basis sets (Fig. 3). This optimized structure of compound 1 was then used for docking studies with AChE and BuChE enzymes.
image file: d0ra02339f-f3.tif
Fig. 3 Optimized structure of compound (1).

Acetylcholinesterase activity

By performing in vitro studies aided with molecular docking simulation it has been observed that compound 1 (% inhibition = 78.92 ± 0.24) is the most active analogue with binding energy −12.9 kcal mol−1 for AChE inhibition activity. In active site, two most important residues Trp286, Trp86, Trp341 and His447 of AChE are frequently involved through hydrogen bonding or π–π interaction and play important inhibitory roles. The compound 1 also involves in hydrogen bonding interaction with Trp124 and Phe295 of AChE. On the basis of docking results it is clear that the compound 1 is able to fit well in active site of acetylcholinesterase and interact with important amino acid residues. The enhanced inhibition activity for compound 1 might be due to the presence of flouro group at ortho position of the extended phenyl ring. The top-ranked docking conformation of compound 1 showed that it is able to form potential π–π interaction with residue Tyr286 and hydrophobic interactions with other residues within the esteratic pocket of the active site (Fig. 4). On the other hand, several amino acid residues appear to stabilize the ground state binding orientation of the phenyl rings by engaging in hydrophobic interaction with the side chains of residues Try124, Gly448 and Phe295 (Table 6).
image file: d0ra02339f-f4.tif
Fig. 4 Binding position for compound (1) in the active site of AChE.
Table 6 Important interactions of compound 1, flurbiprofen and serine with AChE
Compound code Binding energies kcal mol−1 H-bonds interactions Hydrophobic interaction (π–π)
Compound 1 −12.9 Tyr124, Phe295 Trp286, Trp86, Tyr341, His447
Eserine −8.9 Tyr341,
Flurbiprofen −8.3 Phe298 Phe338, Tyr341, Tyr337


Flurbiprofen and eserine were observed to be least active compound having binding score of −10.1 kcal mol−1 and −8.9 kcal mol−1. Binding interactions are shown in Fig. 5 and 6.


image file: d0ra02339f-f5.tif
Fig. 5 Binding position for fIurbiprofen in the active site of AChE.

image file: d0ra02339f-f6.tif
Fig. 6 Binding position for eserine in the active site of AChE.

Butyrylcholinesterase activity

In vitro studies aided with molecular docking simulation revealed that compound 1 (% inhibition = 61.25 ± 0.85) is the active compound with binding energy −9.8 kcal mol−1 compared to eserine (−8.5 kcal mol−1) which is used as standard drug for BuChE inhibition activity and flurbiprofen (−8.2 kcal mol−1). In active site there are two most important residues Trp82 and Tyr332 of BuChE involving through hydrogen bonding or π–π interaction which play an important inhibitory role.

The docking results suggest that compound 1 is able to fit well in active site of butyrylcholinesterase and interact with important amino acid residues. The inhibition activity for compound 1 might be due to the presence of flouro group at ortho position of the extended phenyl ring. Compound 1 showed hydrogen bond interaction with the Thr120 amino acid residue. The top-ranked docking conformation of compound 1 showed that it is able to form potential π–π interaction with residue Tyr332 and hydrophobic interactions with other residues within the esteratic pocket of the active site (Fig. 7). On the other hand, several amino acid residues appear to stabilize the ground state binding orientation of the phenyl rings by engaging in hydrophobic interaction with the side chains of residues Tyr332, Ala328 and Gly116 (Table 7).


image file: d0ra02339f-f7.tif
Fig. 7 Binding position for compound (1) in the active site of BuChE.
Table 7 Important interactions of compound 1, flurbiprofen and eserine with BuChE
Compound code Binding energies kcal mol−1 H-bonds interactions Hydrophobic interaction (π–π)
Compound 1 −9.8 Trp82, Trp332, Thr120 Trp82, Trp332, Ala332, Gly116, Ala328
Eserine −8.5 Tyr128, His438, Phe329 Trp82, Phe329
Flurbiprofen −8.2 Tyr128, His438 Trp82, Ala328, Phe329


Flurbiprofen was observed to be least active compound having binding score −8.2 kcal mol−1. Binding interactions are shown in Fig. 8. Docking and BuChE inhibition activity results revealed that compound 1 could be used for Alzheimer's disease.43


image file: d0ra02339f-f8.tif
Fig. 8 Binding position for fIurbiprofen in the active site of BuChE.

Conclusions

A novel compound (1) based on combination of flurbiprofen and isoniazide has been designed and synthesized. AChE and BuChE inhibition activities of compound (1) were found to be ∼2.5 and ∼1.7 folds higher respectively as compare to standard physostigmine and parent drug i.e. flurbiprofen. Molecular docking studies prove that compound (1) is more active against AChE and BuChE having calculated binding energies of −12.9 kcal mol−1 and −9.8 kcal mol−1 respectively, as compared to flurbiprofen and eserine (physostigmine) for which binding energy was calculated to be −10.1 kcal mol−1 and −8.9 kcal mol−1 respectively. Lineweaver–Burk plot suggests mixed mode of inhibition of AChE and BuChE with compound 1. AChE and BuChE inhibition activities aided with docking results suggested that compound (1) could be used for Alzheimer's disease. Moreover, compound (1) also exhibit improved α-chymotrypsin activity as well. Compound (1) was found to be more active and less toxic than the parent analogues in various in vitro and in vivo tests.

Conflicts of interest

There are no conflicts to declare.

Acknowledgements

We acknowledge University of Education, Township, Lahore, Pakistan for providing laboratory facilities to accomplish this project.

Notes and references

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Footnote

Electronic supplementary information (ESI) available. See DOI: 10.1039/d0ra02339f

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