Kaewta Jetsrisuparbab,
Thanawan Jeejailaa,
Chanon Saengthipa,
Pornnapa Kasemsiriab,
Yuvarat Ngernyena,
Prinya Chindaprasirtbc and
Jesper T. N. Knijnenburg*bd
aDepartment of Chemical Engineering, Khon Kaen University, Khon Kaen 40002, Thailand
bSustainable Infrastructure Research and Development Center, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: jespth@kku.ac.th
cDepartment of Civil Engineering, Khon Kaen University, Khon Kaen 40002, Thailand
dInternational College, Khon Kaen University, Khon Kaen 40002, Thailand
First published on 26th October 2022
The presence of magnesium (Mg) and calcium (Ca) in biochar-based fertilizers is linked to the slow release of phosphorus (P), but these alkali metals have not been systematically compared under identical conditions. In this study, sugarcane filter cake was treated with H3PO4 and MgO or CaO followed by pyrolysis at 600 °C to produce a Mg/P-rich biochar (MgPA-BC) and a Ca/P-rich biochar (CaPA-BC), respectively. The P-loaded biochars were studied by extraction and kinetic release in water over 240 hours to assess the potential P availability. X-ray diffraction and Fourier-transform infrared (FTIR) spectroscopy were used to characterize the pristine and post-kinetics biochars to identify the responsible phases for phosphate release. Additionally, the dissolved P concentrations in the kinetic release experiment were compared to thermodynamic solubility calculations of common Mg and Ca phosphates. Both MgPA-BC and CaPA-BC had P loadings of 73–74 g kg−1 but showed distinctly different release behaviors. Phosphate dissolution from MgPA-BC was gradual and reached 10 g P per kg biochar after 240 hours, with rate-determining phases being Mg2P2O7 (Mg pyrophosphate), MgNH4PO4·6H2O (struvite), and Mg3(PO4)2·22H2O (cattiite). In contrast, CaPA-BC only released 1.2 g P per kg biochar. Phosphate release from CaPA-BC was limited by the low solubility of Ca2P2O7 (Ca pyrophosphate) and (Ca,Mg)3(PO4)2 (whitlockite). Co-pyrolysis with MgO retained P in a more soluble and available form than CaO, making MgO a preferential additive over CaO to immobilize phytoavailable P in biochar-based fertilizers with higher fertilizer effectiveness.
Thailand is the world's 4th largest producer of sugarcane with a production rate of over 100 million tons of sugarcane per year.3 The main solid waste product in the sugar production process is sugarcane filter cake, a nutrient-rich residue that is left behind when cane juice is filtered. Each ton of crushed sugarcane produces 30–40 kg filter cake.4 Due to its high nutrient content (1–2% of each nitrogen (N) and P per dry weight), the filter cake is often directly used as fertilizer,4 but this may result in eutrophication due to losses of water-soluble nutrients like N and P to surface waters.5
Biochar is a porous carbon-rich material that is produced by thermal treatment of biomass in an oxygen-limited environment. Soil application of biochars can provide (long-term) financial gains to farmers,6,7 and the conversion of sugarcane filter cake into a stable biochar presents an attractive solution to convert this waste into a valuable nutrient-rich soil amendment.8–10 Compared to the raw biomass, biochars have a higher P content in a less water-soluble form with reduced leaching.11,12 Especially the presence of calcium (Ca) and magnesium (Mg) aid to immobilize the P into forms that do not rapidly dissolve in water but are still available to crops.13,14 The conversion of a waste biomass such as sugarcane filter cake into a biochar may thus present an attractive slow release P fertilizer.
To further increase the P loading, biochars are frequently treated with a P source either before or after pyrolysis, and especially pre-pyrolysis treatment of the biomass with P in combination with Ca and/or Mg can produce slow release fertilizers.15 Sugarcane leaves treated with MgO and a P source followed by pyrolysis at 600 °C produced biochar-based fertilizers that presented slow P release in water. Without MgO, the acidic P was rapidly released, highlighting the essential presence of Mg.16 Similarly, the presence of Ca and Mg greatly reduced the water solubility of P when poultry litter was co-pyrolyzed with various P sources, with and without the addition of MgO.17 Biochar-based fertilizers synthesized by co-pyrolysis of cotton straw with bentonite and K3PO4 under microwave irradiation had slow release of P and K in soil and were effective in increasing the growth of pepper seedlings in a pot trial.18 Co-pyrolysis of sewage sludge (a P-rich waste) with CaO produced a biochar that could promote the growth of rice seedlings in hydroponic experiments.19 Sewage sludge biochars modified with MgCl2, CaO and MgO (but not CaCl2) were effective at adsorbing P from solution. The Mg-modified biochars had a higher P release rate than the CaO-modified biochar. Adsorbed P was suggested to be rapidly released, whereas the inherent P in the biochars was released more slowly.20 Also the co-pyrolysis of sawdust and switchgrass with triple superphosphate or bone meal at 500 °C produced P-loaded biochars with slow P release kinetics in water.21
In these previous studies, the gradual P release from biochar-based fertilizers has been linked to the presence of poorly soluble Mg and/or Ca phosphate phases. However, the differences in conditions used (e.g., pyrolysis temperature, raw material, and contents of Mg, Ca and P) make it difficult to directly compare the roles of Mg and Ca. Moreover, few studies have investigated the changes in the biochar during phosphate dissolution process,17 and dissolved P concentrations have been rarely compared with thermodynamic solubility values of the phosphates.22 In this work, sugarcane filter cake was pre-treated with H3PO4 and either MgO or CaO, and subsequently pyrolyzed at 600 °C. The P-loaded biochars were studied for their extractable P concentrations in 2% formic acid and deionized (DI) water, and the phosphate release kinetics were evaluated in DI water over 240 hours. To identify the phosphate release mechanism, the pristine and post-kinetics biochars were characterized by X-ray diffraction and FTIR spectroscopy. The proposed formation and dissolution mechanism of the formed Ca/Mg phosphate phases in the biochars supported by thermodynamic solubility calculations is presented.
Three biochars were produced: (i) unmodified filter cake biochar (BC), (ii) filter cake biochar pre-treated with H3PO4 and MgO (MgPA-BC), and (iii) filter cake biochar pre-treated with H3PO4 and CaO (CaPA-BC).
X-ray diffraction (XRD) measurements were carried out on a PANalytical EMPYREAN diffractometer (PANalytical B.V., the Netherlands) with Cu Kα radiation operating at 40 kV and 45 mA. Patterns were collected over 2θ = 5–70° with step size 0.02 s−1. The crystalline phases were identified using X'Pert HighScore Plus software (PANalytical B.V., the Netherlands). Fourier transform infrared (FTIR) spectra were collected on a Bruker Alpha II ATR-FTIR (Bruker, Germany) at 4000–600 cm−1 (32 scans, resolution 2 cm−1). Scanning electron microscopy (SEM) analysis was done on a Hitachi SU3800 (Hitachi, Japan) after sputter-coating the samples with gold.
The total metal (Ca, Mg, Fe, and K) and P contents of the biochars were determined in triplicate after digesting the biochars by modified dry ashing.24 After filtration, the Ca, Mg, Fe, and K concentrations were measured by flame atomic absorption spectroscopy on a PinAAcle 900F (Perkin Elmer, Singapore) after dilution in 1% HNO3. For Ca and Mg measurements, each standard and sample solution contained 1000 ppm Sr to eliminate interferences. The P concentrations were measured by UV-vis spectroscopy (Agilent 8453, Agilent Technologies, USA) via the molybdenum blue method at 880 nm.25
The extractable P of the biochars was evaluated in DI water and 2% formic acid according to the modified method of Wang et al.26 The suspensions (0.3 g biochar in 30 mL extractant) were ultrasonicated for 10 min and placed on an orbital shaker (Gallenkamp, UK) at 120 rpm. After 30 min, the suspensions were filtered through a 0.22 μm nylon filter and the extracted P concentrations were measured by UV-vis spectroscopy via the molybdenum blue method. The pH values of the extracts in DI water were recorded with a digital pH meter (OHAUS Starter 3100, OHAUS Corporation, USA), and the average value for each sample was reported as the biochar pH.16
The following models were used to describe the phosphate dissolution kinetics: zero order, pseudo-first order, pseudo-second order, Elovich, parabolic diffusion, and power function (Table S1 in ESI†). The suitability of each model to describe the release behavior was assessed by the coefficient of determination (R2) and the standard error of the estimate (SE).16
At the end of the kinetic release experiment (after 240 hours) the biochars were collected by filtration (Whatman no. 42, GE Healthcare Life Sciences, UK), rinsed with DI water, dried for 24 hours at 40 °C, and subsequently analyzed by XRD and FTIR spectroscopy as described previously. These post-kinetics biochars are referred to as MgPA-BC-post and CaPA-BC-post. The Ca and Mg contents in the filtrate after 240 hours were measured by AAS as described previously. Additionally, the solution after 240 hours was digested with ammonium persulfate according to the procedure of Huang and Zhang28 with slight modifications. Each solution was mixed with 6 mL DI water and 1.2 mL of a 4.2% ammonium persulfate solution in a 10 mL screw-cap vial. The vials were tightly closed and digestion was carried out in an oven (Biobase BOV-V45F, Biobase Bioindustry Co. Ltd, China) for 16 hours at 90 °C. The P concentration of each sample after persulfate digestion was determined by UV-vis spectroscopy via the molybdenum blue method.
Sample | Yield (%) | pH | Ash (wt%) | Ca (g kg−1) | Mg (g kg−1) | Fe (g kg−1) | K (g kg−1) | P (g kg−1) | P:Mg | P:Ca |
---|---|---|---|---|---|---|---|---|---|---|
BC | 64.3 | 8.1 | 77.5 ± 2.6 | 15.8 ± 1.2 | 2.6 ± 0.2 | 10.9 ± 1.1 | 3.1 ± 0.3 | 12.0 ± 1.1 | 3.7 | 1.0 |
MgPA-BC | 59.9 | 7.7 | 80.9 ± 0.9 | 0.9 ± 0.1 | 40.9 ± 2.7 | 6.0 ± 0.8 | 2.0 ± 0.2 | 74.1 ± 7.3 | 1.4 | 110.5 |
CaPA-BC | 68.4 | 7.0 | 82.6 ± 1.1 | 92.1 ± 4.6 | 2.4 ± 0.1 | 6.9 ± 0.7 | 2.3 ± 0.4 | 73.4 ± 2.6 | 24.2 | 1.0 |
Sample BC contained small amounts of Ca (15.8 g kg−1), Fe (10.9 g kg−1), K (3.1 g kg−1), and Mg (2.6 g kg−1), which were similar to previous studies on sugarcane filter cake biochars.10,32 In the treated biochars, the concentrations of Fe and K decreased to 6.0–6.9 g kg−1 and 2.0–2.3 g kg−1, respectively, likely due to leaching during pretreatment and dilution due to additive addition. As expected, the Mg content in MgPA-BC increased greatly to 40.9 g kg−1. This Mg content was higher than that of sugarcane leaf biochars16 modified with Mg and P but lower than other Mg-modified biochars.17 The Ca content in MgPA-BC conversely decreased to 0.9 g kg−1, which could have been caused by the leaching of Ca in the H3PO4 pre-treatment solution. Upon pre-treatment with CaO and H3PO4, the Ca content in CaPA-BC greatly increased to 92.1 g kg−1, similar to previous works.17,19
The P content of BC was 12.0 g kg−1 which is typical for sugarcane filter cake biochars,10,32 and increased to 73.4–74.1 g kg−1 for the modified biochars. Such P loadings are comparable to previous works.16,17,21,33 The modified biochars had an approximately equimolar ratio of P to additive cation: the P:Mg ratio in MgPA-BC was 1.4, and the P:Ca ratio in CaPA-BC was 1.0.
Biochar morphology was studied by scanning electron microscopy (Fig. 1). The unmodified filter cake biochar (BC) was heterogeneous with particles originating from plant fibers in the sugarcane and angular particles that were likely inorganic compounds.5 Compared to BC, the particle size of MgPA-BC was smaller which may have been a result of the pre-treatment with H3PO4. The MgPA-BC consisted of agglomerates of crystalline particles, which were likely a Mg phosphate such as Mg2P2O7.16 In contrast to MgPA-BC, CaPA-BC was more agglomerated and showed the presence of needle-like or tabular particles, possibly Ca2P2O7.34
Fig. 1 Scanning electron microscopy (SEM) images of (a) BC, (b) MgPA-BC, and (c) CaPA-BC at 1000× magnification. |
Material | SBET (m2 g−1) | Vmicro (cm3 g−1) | VT (cm3 g−1) | Dp (nm) |
---|---|---|---|---|
BC | 32.9 | 0.010 (18%) | 0.054 | 11.7 |
MgPA-BC | 43.6 | 0.011 (14%) | 0.079 | 7.8 |
CaPA-BC | 42.6 | 0.011 (16%) | 0.071 | 9.9 |
Fig. 2 Extractable P from the biochars in DI water (DIW) and 2% formic acid (FA). The values above the bars indicate the percentage of the total P that is extracted with each extraction solution. |
Modification of the biochars greatly increased the FA-extractable P, ranging from 18 g kg−1 (CaPA-BC) to 30 g kg−1 (MgPA-BC). These values accounted for 24–40% of the total P in those materials, in agreement with previous studies.16,26 Compared to Ca, particularly the modification with Mg-containing pre-treatments enhanced the P extractability; the percentage of total P in MgPA-BC that was FA-extractable (40%) was almost twice as large as that of CaPA-BC (24%). This may suggest that the P in MgPA-BC is more available to crops than that in CaPA-BC.
All biochars had a much higher FA-extractable P compared to the DIW-extractable P, which indicated the potential for slow release. Based on these results, both MgPA-BC and CaPA-BC were considered promising materials for slow P release fertilizers and were subsequently tested in a 240 hour release study in water.
Six models were used to assess the phosphate release from the modified biochars (Table S1 in ESI†). Based on the R2 and SE values, the phosphate release from both MgPA-BC and CaPA-BC was best described by the power function (Table 3). In previous studies, phosphate release from other biochars also followed the power function,21,22 but also parabolic diffusion,16,21 Elovich,17 and even zero order equation12 have been used. The constants for the power function were a = 1.065 g kg−1 h−0.42 and b = 0.42 (MgPA-BC), and a = 0.478 g kg−1 h−0.20 and b = 0.20 (CaPA-BC). For both biochars, the values of b were less than 1, implying that the release rates decreased with time. For MgPA-BC, b was close to 0.5, in which case the power function approaches the parabolic diffusion model; indeed, the parabolic diffusion model provided the second best fit (Table 3). The a value represents the initial release rate, which indicated that MgPA-BC had an initial phosphate release rate that was more than twice as large as that of CaPA-BC. To identify the dissolution mechanism, the biochars were characterized by X-ray diffraction and FTIR spectroscopy before and after the kinetics dissolution experiments, and the results are presented in the next sections.
Model | MgPA-BC | CaPA-BC |
---|---|---|
R2/SE | R2/SE | |
Zero order | 0.87/1.46 | 0.67/0.23 |
Pseudo-first order | 0.97/0.55 | 0.75/0.19 |
Pseudo-second order | 0.97/0.97 | 0.99/0.19 |
Elovich | 0.90/1.25 | 0.97/0.07 |
Parabolic diffusion | 0.98/0.55 | 0.87/0.15 |
Power function | 1.00/0.43 | 0.99/0.08 |
The pristine Ca-containing pre-treated biochar (CaPA-BC) contained crystalline Ca pyrophosphate (γ-Ca2P2O7), as also seen in previous studies where P-loaded biochars were formed by pre-treatment with Ca and P.17,21,33 After the kinetic study, peaks for Ca2P2O7 were still present in CaPA-BC-post but reduced in intensity, and new peaks corresponding to (Ca,Mg)3(PO4)2, a Mg-stabilized β-Ca3(PO4)2 phase sometimes referred to as whitlockite, were found at 2θ = 17.0°, 28.2°, 31.5°, 34.9°, 47.4° and 48.9°.
Fig. 5 FTIR spectra (1800–600 cm−1) of pristine BC and MgPA-BC and CaPA-BC, and the modified biochars after the 240 hour kinetic release experiment (MgPA-BC-post and CaPA-BC-post, respectively). Approximate peak positions are indicated above each peak. The FTIR spectra over the full range (4000–600 cm−1) can be found as Fig. S1 in the ESI.† |
Upon pre-treatment with MgO and H3PO4, the FTIR spectrum of MgPA-BC showed a relative increase in peak intensity around 1090 cm−1 compared to BC, possibly because of incorporation of a (pyro)phosphate,36,40 likely Mg2P2O7 as observed in the XRD patterns (Fig. 4). Possibly, the shoulder peak at 917 cm−1 can also be ascribed to Mg2P2O7.41 The increased intensity of a peak around 951 cm−1 indicated the presence of symmetric stretching mode of P–O bonds of orthophosphate groups,42 and the shoulder at 880 cm−1 was ascribed to HPO4 group vibrations.43 However, more information about the presence of specific phosphate phases in MgPA-BC was difficult to obtain due to the overlap of the phosphate vibrations with the Si–O peaks in the region 800–1300 cm−1.
After the kinetic experiment, the peaks at 1045, 951, and 880 cm−1 decreased in intensity, possibly suggesting the dissolution of an orthophosphate phase. As a result, the broader underlying peak with maximum at 927 cm−1 was found in MgPA-BC-post (this peak was present in MgPA-BC but masked by the peak at 951 cm−1 and only found as a shoulder at 917 cm−1), which was ascribed to Mg2P2O7.41 At lower wavenumbers (600–800 cm−1) peaks have become broader, possibly due to the formation of struvite36 that has an absorbance peak at 770 cm−1 that overlapped with the vibrational peaks of quartz. The cattiite (Mg3(PO4)2·22H2O) that was identified by XRD has its main absorbance peak43 at 980 cm−1 but this peak could not be confirmed with FTIR spectroscopy due to the low quantity and overlap with other quartz/phosphate peaks.
In the FTIR spectrum of CaPA-BC, the peaks at 611, 719, 935, 998, 1034, 1075, 1137, 1159, and 1194 cm−1 were assigned to γ-Ca2P2O7.44 The peak at 884 cm−1 indicated the presence of HPO4 groups from amorphous CaHPO4.45 After the kinetic experiment, there was only little change in the FTIR spectrum of CaPA-BC-post compared to CaPA-BC. Various peaks have broadened, which may suggest a reduction in crystallinity and potentially a transformation of phosphate phases. Moreover, the HPO4 peak at 884 cm−1 reduced in intensity and broadened to become a shoulder peak, possibly indicating its dissolution. The (Ca,Mg)3(PO4)2 as identified in the XRD pattern (Fig. 4) has its main FTIR absorbance peaks in the region 970–1120 cm−1,46 but the presence of these peaks could not be confirmed in CaPA-BC-post due to the overlap with the Ca2P2O7 and SiO2 vibrations.
MgO + H3PO4 + 2H2O → MgHPO4·3H2O | (1) |
(2) |
(3) |
Before pyrolysis, the reaction between MgO and H3PO4 in the pre-treatment process resulted in the formation of MgHPO4·3H2O.47 Upon thermal treatment, dehydration of MgHPO4·3H2O took place around 120–170 °C and resulted in an amorphous MgHPO4 phase.48,49 Further heating converted this amorphous phase into crystalline α-Mg2P2O7 that is formed around 500–600 °C depending on the particle size and heating conditions.49,50 The conversion of amorphous MgHPO4 into crystalline α-Mg2P2O7 follows a complicated mechanism of various polyphosphate chain lengthening and shortening steps.50
However, Mg2P2O7 was likely not the only P form in MgPA-BC. Aramendía et al.51 estimated with solid-state 31P MAS NMR measurements that when MgHPO4·3H2O was heated to 650 °C the material consisted of a mixture of 15% crystalline Mg3(PO4)2 and 85% crystalline α-Mg2P2O7. At a lower temperature of 500 °C, the Mg3(PO4)2 was amorphous and its proportion was larger than at 650 °C (i.e., >15%). These data suggested that MgPA-BC, which was produced at 600 °C, likely contained a substantial fraction of amorphous Mg3(PO4)2 in addition to the crystalline α-Mg2P2O7. This was also inferred from the presence of an orthophosphate peak at 951 cm−1 in the FTIR spectrum of MgPA-BC (Fig. 5). In addition, other (amorphous) orthophosphate phases may have been present as well. Possibly, some struvite (MgNH4PO4·6H2O) and/or struvite-K (MgKPO4·6H2O) may have been formed by the presence of respectively NH4+ and/or K+ in the biomass.47 Even though struvite is unstable at high temperatures (the loss of water and NH3 from struvite takes place41 below 300 °C), Bekiaris et al.36 found indications that an amorphous struvite phase was present in biochar produced from a solid fraction of digestate at 600 °C. The presence of struvite in biochars was also suggested by Bruun et al.11 from the co-occurrence of Mg and P in SEM-EDX analysis with high spatial correlation, and also Wang et al.12 suggested that a Mg phosphate mineral (possibly struvite) was the main phase responsible for P release from poultry litter biochar. Similarly, struvite-K was previously identified in various biochars.17,52 However, the presence or absence of struvite or struvite-K in MgPA-BC could neither be confirmed nor be ruled out. Possibly, some of these phases may have been present in MgPA-BC, but were masked by other peaks in the XRD pattern and FTIR spectrum. The largely amorphous nature of MgPA-BC and the presence of SiO2 made it difficult to confirm the specific orthophosphates.
Based on the identified phases in XRD and FTIR analysis, the phosphate release from MgPA-BC could be separated into two processes, namely: (1) dissolution of crystalline Mg2P2O7, and (2) dissolution of an amorphous orthophosphate (such as struvite, struvite-K, and/or Mg3(PO4)2). In the XRD pattern of MgPA-BC, α-Mg2P2O7 was the sole crystalline phosphate phase, and the intensity decreased during the dissolution process that suggested its dissolution. We could not find any reliable Ksp values for Mg2P2O7, but it can be inferred that Mg2P2O7 is more soluble than Ca2P2O7 since the presence of Mg2+ ions was reported to increase the Ca2P2O7 solubility.34,53 The dissolution of Mg2P2O7 likely took place via the release of pyrophosphate (P2O74−) ions, which likely remained in solution as pyrophosphate ions, since the conversion of pyrophosphate to orthophosphate takes place only very slowly at room temperature and neutral pH.34 To confirm the release of condensed phosphates (such as pyrophosphates but also longer chains) from MgPA-BC, the solution after the 240 hour kinetic release experiment was digested with a persulfate solution (Table S3 in ESI†). After persulfate digestion, the dissolved P concentration after 240 hours was 68.4 ± 3.3 mg L−1, which is higher than the P concentration before persulfate digestion (49.6 ± 1.4 mg L−1), indicating that indeed some Mg2P2O7 dissolved. It should be noted that the P dissolution from Mg2P2O7 cannot explain the phosphate release kinetics because the dissolved P concentrations in Fig. 3a consist primarily of orthophosphates.54
Thus, in addition to Mg2P2O7 (the only crystalline phosphate phase), an (amorphous) orthophosphate also dissolved from MgPA-BC. To identify the determining phase for phosphate dissolution, the dissolved P concentrations from MgPA-BC were compared with calculated solubility values of various common Mg phosphates (Fig. 6). After 240 hours, struvite and Mg3(PO4)2·22H2O appear to be the rate-limiting phases for long-term phosphate dissolution; the dissolved P concentration agreed very well with the calculated solubility of struvite and Mg3(PO4)2·22H2O (see Fig. 6). Indeed, struvite/struvite-K and Mg3(PO4)·22H2O were the phases that appeared in the XRD pattern of MgPA-BC-post (Fig. 4). In previous experiments, struvite was in equilibrium with Mg3(PO4)2·22H2O under similar alkaline conditions.55 The dissolved Mg concentration after 240 hours (34.3 ± 1.5 mg L−1) was similar to the dissolved P concentrations (Table S3 in ESI†), which further confirmed that an Mg-containing phosphate dissolved from the biochar.
Amorphous Mg3(PO4)2 was possibly also present in MgPA-BC as suggested by the FTIR spectrum (Fig. 5) and previous work.51 It is likely that the amorphous Mg3(PO4)2 converted to crystalline Mg3(PO4)2·22H2O during the kinetic release experiment.
CaO + H3PO4 + H2O → CaHPO4·2H2O | (4) |
(5) |
(6) |
The reaction of CaO with H3PO4 resulted in the formation of CaHPO4·2H2O.23 Upon heating, the CaHPO4·2H2O was dehydrated to form CaHPO4 at around 150–160 °C, which further converted into crystalline γ-Ca2P2O7 at around 350–550 °C.50,56
Apart from Ca2P2O7, other Ca phases may have been present as well, either as amorphous or small amounts of crystalline phases. Examples of phases that were previously observed in biochars prepared under similar conditions were CaHPO4,11,17,36 Ca3(PO4)2, Ca8H2(PO4)6·5H2O,22 and hydroxyapatite (Ca10(OH)2(PO4)6).17,22 When comparing the solubility of these phases with the dissolved P concentrations from CaPA-BC (Fig. 7), it appears that β-Ca3(PO4)2 (i.e., (Ca,Mg)3(PO4)2) was the rate controlling phase, in agreement with the XRD pattern of CaPA-BC-post (Fig. 4). In addition, a more soluble phase such as (amorphous) CaHPO4 was likely also present in CaPA-BC, as was suggested by the HPO4 peak at 884 cm−1 in the FTIR spectrum of CaPA-BC (Fig. 5) and since this phase was identified in various Ca- and P-rich biochars.11,17,36
Based on the identified phases, the phosphate dissolution from CaPA-BC was separated into two processes, namely (1) dissolution of crystalline Ca2P2O7, and (2) dissolution of an amorphous orthophosphate (such as (Ca,Mg)3(PO4)2 and/or CaHPO4). The solubility of Ca2P2O7 is very low, with reported Ksp values ranging from 3 × 10−18 to 1.8 × 10−13.53,57 Little to no dissolution of Ca2P2O7 was also confirmed by the fact that the dissolved P concentrations before and after persulfate digestion were not different (Table S3 in ESI†). Lustosa Filho et al.17 also found that crystalline Ca2P2O7 was still present after their 240 hour kinetic release experiment of poultry litter biochars.
Similar to MgPA-BC, the phosphate dissolution from CaPA-BC cannot be solely ascribed to the formation of Ca2P2O7, and an orthophosphate phase must have been responsible for the P release. The dissolved P concentrations did not change any further after 144 hours (Fig. 3a) and equilibrium was reached. When comparing the dissolved P concentrations with the calculated solubilities of various Ca phosphates, the equilibrium P concentration was in good agreement with the calculated solubility of β-Ca3(PO4)2 (Fig. 6b). This was further corroborated by the presence of (Ca,Mg)3(PO4)2 in the XRD pattern of CaPA-BC-post (Fig. 4). In previous studies, the slow phosphate release and poor solubility of P and Ca from various biochars was also ascribed to crystalline (Ca,Mg)3(PO4)2.22,58 It should be noted that the actual solubility of (Ca,Mg)3(PO4)2 may be lower than that of the calculated Mg-free β-Ca3(PO4)2, depending on the Mg content.46
After 240 hours, close to 2% of the P present in CaPA-BC dissolved, and only 0.5% of the total Ca has dissolved (Table S3 in ESI†). This very low amount of Ca dissolved suggested that an additional phase different than CaHPO4 or (Ca,Mg)3(PO4)2 may have been responsible for P dissolution and/or the Ca may have reprecipitated on the biochar surface in the form of (Ca,Mg)3(PO4)2.
Footnote |
† Electronic supplementary information (ESI) available: Equations for kinetics release, solubility products (Ksp), and dissolved concentrations (P before and after persulfate digestion, Mg, Ca) after 240 hours. See DOI: https://doi.org/10.1039/d2ra05848k |
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