Lorenzo Francesco Madeo†
*a,
Christine Schirmer†b,
Giuseppe Cirilloc,
Samuel Froeschkea,
Martin Hantuscha,
Manuela Curcioc,
Fiore Pasquale Nicolettac,
Bernd Büchnerad,
Michael Mertigbe and
Silke Hampela
aLeibniz Institute for Solid State and Materials Research Dresden, Dresden 01069, Germany. E-mail: l.f.madeo@ifw-dresden.de; Tel: +49 3514659883
bKurt-Schwabe-Institut für Mess- und Sensortechnik Meinsberg e.V., Kurt-Schwabe-Straße 4, Waldheim 04736, Germany
cDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, Rende 87036, CS, Italy
dInstitute of Solid State and Materials Physics, Technische Universität Dresden, Dresden, 01062, Germany
eInstitute of Physical Chemistry, Technische Universität Dresden, Dresden, 01062, Germany
First published on 11th September 2023
Zinc oxide/Curcumin (Zn(CUR)O) nanocomposites were prepared via hydrothermal treatment of Zn(NO3)2 in the presence of hexamethylenetetramine as a stabilizing agent and CUR as a bioactive element. Three ZnO:CUR ratios were investigated, namely 57:43 (Zn(CUR)O-A), 60:40 (Zn(CUR)O-B) and 81:19 (Zn(CUR)O-C), as assessed by thermogravimetric analyses, with an average hydrodynamic diameter of nanoaggregates in the range of 223 to 361 nm. The interaction of CUR with ZnO via hydroxyl and ketoenol groups (as proved by X-ray photoelectron spectroscopy analyses) was found to significantly modify the key properties of ZnO nanoparticles with the obtainment of a bilobed shape (as shown by scanning electron microscopy), and influenced the growth process of the composite nanoparticles as indicated by the varying particle sizes determined by powder X-ray diffraction. The efficacy of Zn(CUR)O as anticancer agents was evaluated on MCF-7 and MDA-MB-231 cancer cells, obtaining a synergistic activity with a cell viability depending on the CUR amount within the nanocomposite. Finally, the determination of reactive oxygen species production in the presence of Zn(CUR)O was used as a preliminary evaluation of the mechanism of action of the nanocomposites.
Zinc oxide nanoparticles (ZnO NPs) showed remarkable properties as an emerging semiconductor material with a wide range of applications, including biomedicine, due to their biocompatible nature.6 Zinc is a trace mineral, essential for the activation of several enzymes with a role in cell growth, protein and DNA synthesis, and tissue healing.14 Likewise, ZnO NPs are biocompatible towards mammalian cells due to their low dissolution rate under physiological conditions (pH = 7.4), while they become toxic at slightly acidic pH as consequence of rapid release of Zn2+ ions, causing oxidative stress and cell damage.15 Since acidification of the extracellular medium is a hallmark of the tumour environment,16 this feature plays a key role in selective cancer toxicity. ZnO NPs have been employed as both drug carrier and active toxic agent towards TNBC cell lines,17 with morphology and size playing a key role in their efficacy. ZnO NPs have been synthesized with various methods, such as thermal decomposition, sol–gel routes, hydrothermal methods, ultrasonication and green synthesis.18 The resulting particles can be functionalised with polymers to enhance hydrophilicity and drug encapsulation efficiency, and displayed a variety of morphologies, including spheres, dumbbells, rods, flowers, tubes, and hexagonal prisms.19
Alongside conventional chemotherapeutic molecules, a growing number of naturally occurring molecules have been identified as potential candidates for cancer treatment. Among these, curcumin (CUR), a diferuloylmethane constituent of the yellow pigments isolated from Curcuma longa, has been extensively investigated for its potent anti-proliferative effects due to its ability to interfere with different cellular pathways involved in carcinogenesis.20,21 CUR has been tested against a variety of cancers, including prostate cancer,22 squamous cell carcinoma,23 colorectal cancer,24 glioblastoma,25 and breast cancer.26 CUR has demonstrated anti-proliferative activity on cell cycle regulatory proteins, matrix metalloproteinases and NF-κB in the TNBC cell line MDA-MB-231,27 as well as inhibitory activity on the inflammatory cytokines CXCL1/2, resulting in inhibition of the expression of a number of metastasis-promoting genes.28 While various phase I clinical trials have reported that CUR is well tolerated even at high doses, its reduced bioavailability has been the major drawback for its therapeutic use.29 Researchers have therefore attempted to overcome these obstacles through functionalisation, chemical modification, nanoparticles formulation, and the use of adjuvants to interfere with CUR metabolism.30 Metal oxide particles, such as ZnO NPs, can provide a suitable delivery system to overcome the limitations of CUR as a pure therapeutic agent,31 and at the same time exert their own biological activity, resulting in a synergistic effect.
In this study, ZnO and CUR have been combined in a stable composite (Zn(CUR)O), through a one-pot, straightforward hydrothermal synthesis. Zn(NO3)2 has been used as source of Zn2+ ions for ZnO NPs, while hexamethylenetetramine (HMTA) in aqueous solution has provided a weak basic environment and has stabilized Zn2+ during the reaction.32 The conjugation with three different concentrations of CUR affected the ZnO NPs morphology and crystallite size, shifting from hexagonal prisms to roughly dumbbell-shaped aggregates of nanosized crystals. Zn(CUR)O composites were tested in terms of toxic effect on both MCF-7 and MDA-MB-231 breast cancer cells, and the production of reactive oxygen species (ROS) over time was investigated as the main biological mechanism.
Scanning electron microscope (SEM) images of the samples were obtained using a NOVA NanoSEM 200 [5–15 kV] (Thermo Fisher Scientific, Hillsboro, OR, USA) by deposition of previously water-dispersed samples on Si/SiO2 substrates (Plano GmbH, Wetzlar, Germany).
Dynamic light scattering (DLS) analyses were performed with a 90 Plus Particle Size Analyzer (Brookhaven Instruments Corp, Holtsville, NY, USA) at 25.0 ± 0.1 °C with a laser operating at 658 nm while measuring the autocorrelation function at 90°.
Fourier transform infrared spectroscopy (FTIR) spectra were recorded using a Vertex80v (BRUKER Optic GmbH, Karlsruhe, Germany) with KBr pellet technique, with 250 scans performed for each spectrum.
Photoelectron spectroscopy (XPS) was carried out using a PerkinElmer PHI-5600 spectrometer equipped with a 150 mm hemispherical analyser (PerkinElmer, Inc., Waltham, MA, USA). A non-monochromatic Mg-Kα X-source (250 W) was chosen and the data is satellite corrected. The powder samples required a special geometry which leads to an electron emission angle of 45° relative to the analyser. A neutraliser is used to avoid charging effects and the binding energy scale is corrected based on the shift of C 1s graphitic carbon at 284.8 eV for each spectrum. High resolution spectra are carried out with a 29.35 eV pass energy.
Thermogravimetric analysis (TGA) was performed on an SDT Q600 (TA Instruments, Hüllhorst, Germany) under a nitrogen atmosphere with the following conditions: 2 mg initial sample weight, 10 mL min−1 N2 flow, 25–800 °C temperature range, and 10 °C min−1 heating rate.
For diffuse reflection spectroscopy, samples were previously pressed onto BaSO4 pellets and measured with a Shimadzu UV-3101PC Spectrometer with an attached multi-purpose large-sample compartment MPC-3100.
For determining cytotoxic activity and ROS-inducing potential of the synthesised Zn(CUR)O composites, ZnO NPs and pure CUR, samples were pre-diluted in cell culture grade DMSO (PAN Biotech, Aidenbach, Germany) and afterwards dissolved in media without phenol red without or with serum.
Cells were seeded in 96-well plates at 5 × 105 cells per well (MCF-7) or 4 × 105 cells per well (MDA-MB-231), grown to 70–80% confluence and treated with the composites as well as controls for 48 h.
Microscopy images of the cells were taken after treatment using an Axio observer-Z1 microscope (Carl Zeiss Microimaging, Jena, Germany) equipped with an Evolve-EM 512 camera (Photometrics, Tucson, AZ, USA).
(1) |
Abs = (Abs480 − Abs630)no Trit − (Abs480 − Abs630)Trit | (2) |
Therewith, possible interferences from nanoparticles with the absorbance readings and/or the WST-1 substrate are considered.
(3) |
For ZnO NPs, zinc nitrate provides Zn2+ ions, while HMTA is supposed to play multiple roles in the reaction. Due to its non-ionic cyclic tertiary amine structure, it is thought to act as bidentate Lewis base, coordinating two Zn2+ ions.39 HMTA hydrolyses in water, releasing NH3 and providing a weak basic environment for Zn2+ to form Zn(OH)2, which eventually dehydrates to ZnO. The series of reactions can be summarised as follows (eqn (4)):
(4) |
The ZnO growth mediated by HMTA aqueous solution was preferred over growth in strong alkaline solutions in order to partially prevent the CUR degradation at extremely high pH.40 The addition of pure CUR at three different concentrations to the reaction environment conferred an orange colour to the previously white ZnO powder, obtaining Zn(CUR)O-A, Zn(CUR)O-B and ZnO(CUR)O-C. Several different concentrations of CUR were tested beforehand. The lowest concentration chosen resulted to be the minimum for obtaining a significant modification of the ZnO properties. On the other hand, further increases in CUR concentration above the highest used in this work did not show substantial differences in terms of composite composition and toxic activity towards cancer cells (see below).
The repeated centrifugation steps with acetone washed away the excess of CUR that was adsorbed on the ZnO surface.
The persistent orange colour of the powder was an indication of a significant chemical bonding between the CUR and the ZnO, forming a composite.
In order to confirm the purity of the samples, investigate their crystal structure, and determine the crystallite size, pXRD was performed on pure ZnO and Zn(CUR)O composites (Fig. 2).
The presence of all the ZnO hexagonal phase diffraction peaks reported in the literature confirmed the hexagonal ZnO wurtzite structure of all the samples analysed. The absence of any additional peaks demonstrated the crystalline purity of the samples. Furthermore, no CUR peaks were observed, indicating the amorphous nature of curcumin in the composites.
At the same time, a progressive broadening of the peaks was observed as the CUR content increased from pure ZnO NPs to Zn(CUR)O-A. This was associated with a decrease in crystallite size to nanoscale dimensions.41 By the Scherrer equation, the equatorial crystallite sizes were calculated to be 104.6 ± 0.7 nm for ZnO, 12.6 ± 0.1 nm for Zn(CUR)O-A, 15.6 ± 0.1 nm for Zn(CUR)O-B and 47.1 ± 0.2 nm for Zn(CUR)O-C, while values of 106.1 ± 1.2 nm for ZnO, 28.4 ± 0.3 nm for Zn(CUR)O-A, 33.3 ± 0.3 nm for Zn(CUR)O-B and 66.7 ± 0.6 nm for Zn(CUR)O-C were obtained for the axial size. The results suggest that CUR in amorphous form interacts with crystalline ZnO without affecting its crystalline structure, but hinders its growth by capping the outer faces, slowing down the overall growth process and ultimately reducing the crystallite sizes.
SEM was used to investigate the morphology of the Zn(CUR)O with different CUR content, in comparison to the pure ZnO NPs (Fig. 3). To get a better image of the surface morphology of the particles, larger particles were considered. ZnO exhibited a typical growth morphology of hexagonal pillars for ZnO.42 In contrast with the methods involving substrate for crystal nucleation, here the particles have wider dimensions in all three dimensions. In presence of CUR, ZnO crystal agglomerates in bilobed shaped form (Fig. 3b–d). At low concentrations of CUR (Zn(CUR)O-C), the smooth surface of ZnO is still visible, while less defined surface shapes were observed at higher concentrations of CUR (Zn(CUR)O-A and B).
To assess the applicability of the synthesized nanocomposites in biological systems, their sizes and the size distributions were measured in DIW by DLS analyses.
The hydrodynamic diameter (HD) was clearly affected by the presence of CUR in the composite. Zn(CUR)O-A was found to have the lowest HD (223 nm, Polidispersivity index – PDI = 0.14), with an evident drop of size compared to the pure ZnO NPs (440 nm, PDI = 0.4). Intermediate values were found for Zn(CUR)O-B (245 nm, PDI = 0.32) and Zn(CUR)O-C (361 nm PDI = 0.24). These results proved the effective obtainment of nanosystems within the accepted range for biomedical applications.43–45
FTIR spectra of ZnO, CUR and their composites (Zn(CUR)O-A, Zn(CUR)O-B and Zn(CUR)O-C) are shown in Fig. 4. A relevant absorption peak in the range of 400–500 cm−1 was observed in the spectra of pure ZnO and Zn(CUR)O composites, which was assigned to the characteristic stretching vibration of the Zn–O bond.46
A rounded broad peak at 3450 cm−1 in the spectra of pure CUR and Zn(CUR)O composites is related to the phenolic O–H stretching vibration. In the same region, a similar peak is also present to a lesser extent in the spectrum of ZnO, which is most likely due to residual hydroxyl groups on the surface of the oxide structure.
With respect to CUR, peaks were found for aromatic CC stretching (1626 cm−1), benzene ring stretching (1588 cm−1), CO vibrations (1510 cm−1), olefinic C–H bending vibrations (1427 cm−1) and aromatic C–O stretching vibrations (1280 cm−1). Spectra of Zn(CUR)O composites showed the same peaks, with slight broadening and shifts for the CO vibration peaks (1504 cm−1), which can be related to the interaction of Zn2+ ions with keto-enolic moieties of CUR. FTIR data confirmed the presence of both CUR and ZnO in every composite. In general, Zn(CUR)O-C showed more pronounced signals related to ZnO than Zn(CUR)O-A and Zn(CUR)O-B, while more pronounced signals related to CUR were observed in Zn(CUR)O-A.
XPS analysis was performed to investigate the surface chemical composition of CUR, ZnO and Zn(CUR)O composites (Fig. 5). The binding energies were calibrated by taking the C 1s peak (284.8 eV) as reference. In the Zn 2p3/2 region (Fig. 5a), ZnO showed a peak at 1021.5 eV, related to ZnO, whose binding energy shifts are reported to be in the same range (1021 eV–1023 eV).47 In the Zn(CUR)O-C spectrum a slight shifting to 1021.69 eV was observed, while in the Zn(CUR)O-A and Zn(CUR)O-B spectra a further shift and an evident broadening (1022.32 eV) suggest a more inhomogeneous chemical environment around the Zn atoms. This is related to the coordination of Zn to the keto-enolic group of CUR, as reported elsewhere in the literature.48 In the O 1s region (Fig. 5b), the peak at 530.2 eV corresponds to the O2− of the wurtzite structure of ZnO.49 In the spectra of Zn(CUR)O-A and Zn(CUR)O-B, very broad signals at higher binding energies can be ascribed to the presence of multiple groups, such as CO and Zn–OH.50 These peaks are in good agreement with the hypothesis that Zn interacts with CUR moieties, such as hydroxyl and keto-enol groups.
Thermogravimetric analyses were performed to evaluate the thermal stability of the composites under analysis, compared with ZnO and CUR, and their composition ratios (Fig. 6). As expected, ZnO is thermally stable up until 800 °C, showing only a minor loss of weight (3.7%) potentially due to the thermal decomposition of residual zinc hydroxide.51 CUR showed multiple stages of thermal decomposition. A first weight loss starts at 170 °C, related to dehydroxylation. For the Zn(CUR)O composites this stage is shifted and less prominent, suggesting a stabilising effect mediated by Zn interaction with –OH group of CUR. At 60 °C, Zn(CUR)O-C exhibits a one-step weight loss (2%), related to the elimination of crystalline water,52 while Zn(CUR)O-A and Zn(CUR)O-B shows a gradual weight loss (7%) until 290 °C. The subsequent degradation stage of CUR starts at 320 °C, involving the decomposition of other groups such as benzene rings.53 This stage is shifted at 365 °C for the Zn(CUR)O composites. Overall, the results highlight that an increase in the ZnO content of the composites led to a minor loss in weight, indicating higher thermal stability.54 Since the same amount of ZnO was used for the synthesis of all the composites, by mean of the TGA weight percentage loss at 800 °C, it was possible to calculate the ZnO:CUR ratios of Zn(CUR)O composites, which resulted to be 57:43 for Zn(CUR)O-A, 60:40 for Zn(CUR)O-B and 81:19 for Zn(CUR)O-C.
Despite the double amount of CUR in the initial reactions of Zn(CUR)O-A compared to Zn(CUR)O-B, the two composites resulted to have similar composition ratios, further suggesting that ZnO is only capable of binding a limited amount of CUR and that increase in CUR leads to a saturation of the CUR amount of the composite particles.
Some previous researches raised the hypothesis that band gap shifts of metal oxides at biologically relevant energies may be related to ROS production.55 A previously proposed theoretical model claimed the possibility to predict the oxidative stress potential of oxide nanoparticles towards biological material, by checking if the conduction band of such oxides is superimposing on the standard redox potentials of couples active in biological media, ranging between −4.12 eV and −4.84 eV.56 The conduction band of ZnO lies higher with respect to the mentioned range, suggesting that its toxicity is mainly related to solubility and ion leaching.57 Nevertheless, it is possible to consider that reducing the band gap of ZnO may increase the chances of having band energy values comparable to the interesting range. In order to investigate whether the synthesised Zn(CUR)O composites present any band gap shift with respect to the pure ZnO, their optical band gaps were examined by diffuse reflection spectroscopy. By transforming the obtained data with the Kubelka–Munk function, they were fitted with the Tauc plot method (γ = 1/2 for direct band gap) as shown in Fig. 7. The optical band gap of ZnO was found to be 3.07 eV, a value in good agreement with rod-shaped nanoparticles,58 while for pure CUR the plot resulted in a band gap of about 2.20 eV.
Fig. 7 Tauc plot of the diffuse reflection spectroscopy results with applied tangents for bandgap determination of CUR, ZnO NPs and Zn(CUR)O nanocomposites. |
For the Zn(CUR)O composite materials, the determination of the exact optical band gap is more difficult, as the spectra show additional Urbach tails, most likely as a result of the amorphous nature of the CUR part of the composite. When accounting for that, the absorption spectra and the determined optical band gaps are significantly different from those of pure CUR and ZnO and are not a simple overlapping of the two individual spectra. Hereby the spectra of Zn(CUR)O-A and Zn(CUR)O-B are again quite similar, while Zn(CUR)O-C shows a slightly higher optical band gap that is still different from the pure components. This clearly suggests an interaction between the CUR shell and the ZnO core of the composite materials, while red-shifting the band gap and adding the possibility for adsorption in the red and near infrared energy range.
Toxicity profiles were evaluated in terms of cell viability after 48 h incubation, testing four different concentrations for each sample (50, 25, 12.50 and 6.25 μg mL−1). As shown in Fig. 8a, both ZnO and CUR did not show any toxic effect over the whole range of concentrations tested, with the exception of a very small decrease of viability in cells treated with the highest concentration of ZnO. For the Zn(CUR)O composites, at the tested incubation time the toxicity was concentration dependent, while hardly any effect was measured at the concentrations of 12.50 and 6.25 μg mL−1 for all the three composites, Zn(CUR)O-A and Zn(CUR)O-B showed a remarkable efficacy starting from the concentration of 25 μg mL−1, reducing the cell viability to 11% and 34%, respectively.
At the highest concentration (50 μg mL−1), cells incubated with Zn(CUR)O-A and Zn(CUR)O-B showed no viability, while the survival rate of those treated with Zn(CUR)O-C was reduced to 50%. A clear role of the CUR content in the toxicity of Zn(CUR)O was demonstrated by the reported data, with a decreasing survival rate of the cancer cells associated with increasing CUR levels within the composites. According to the previous calculation of Zn:CUR ratios, the CUR contents in Zn(CUR)O-A, Zn(CUR)O-B and Zn(CUR)O-C (21.5, 20.0 and 9.5 μg mL−1, respectively, when the particles concentration is 50 μg mL−1) fall within the concentration range of pure CUR tested, which showed no effect. Therefore, a clear improvement of cytotoxic activity was achieved by a synergistic effect between ZnO and CUR in the synthesised composite.
Our data are consistent with previous research, showing that ZnO does not exert any cytotoxic activity on MCF-7 cells at the concentrations employed in our study, while it has anticancer activity towards the cells at higher concentrations.59
After observing a clear toxic effect of Zn(CUR)O on MCF-7 cells, the obtained composite was tested against MDA-MB-231, a highly aggressive and invasive TNBC cell line. As for the previously treated cell line, Fig. 8b shows that CUR has no toxic effect at any of the concentrations tested. ZnO NPs only showed a reduction in cell viability (71%) at the highest concentration used. Considering the composites, Zn(CUR)O-C showed a reduction in cancer cell viability of 46% at 25 μg mL−1 and 99% at 50 μg mL−1. Zn(CUR)O-A and Zn(CUR)O-B showed 52% and 84% cell viability, respectively, after 48 hours of incubation at the lowest concentration used, while comparable results were observed at higher concentrations, with complete killing of treated cells already at a concentration of 25 μg mL−1.
The findings obtained by the cytotoxicity assay are adequately reflected by microscopy images shown in Fig. 9. Here, cell morphology at 25 μg mL−1 and 12 μg mL−1 is shown for the MCF-7 and MDA-MB-231 cells, respectively. For both cell lines, control cells as well as ZnO- and CUR-treated cells showed normal morphology and adherent growth. With Zn(CUR)O-C, cells started to alter morphology and detached from the bottom of the cell culture flasks, which are indicators for beginning cytotoxic reaction. Clear signs for cell death can be seen with Zn(CUR)O-B and Zn(CUR)O-A with higher CUR contents in the composites, especially for the MDA cells, where most of the cells display a rounded shape and were completely detached from the bottom.
Overall, a more pronounced effect of the Zn(CUR)O composites was observed against less treatable cancer cells such as MDA-MB-231 compared to the MCF-7 cell line. Such differential responses were also found for pure CUR by Jia et al.60 who established the involvement of a specific signalling pathway in the mechanism of action of CUR.
Comparing the results with the available data in literature60–64 regarding the individual anticancer effect of ZnO and CUR towards both MCF-7 and MDA-MB-231, here an improved synergistic effect between ZnO and CUR was demonstrated in the synthesised composite Zn(CUR)O. To explain the toxicity exerted by Zn(CUR)O particles, the hypothesis of induced oxidative stress towards cancer cells has been investigated (Fig. 10). In particular, the production of ROS (e.g. O2˙−, OH•, H2O2) by ZnO and Zn(CUR)O samples was measured by DCFDA technique after 4 h (as per protocol) at the maximum concentration used in viability assays (50 μg mL−1). CUR was excluded from these tests, because its own fluorescence emission produced a high signal, which was confirmed by tests performed without treatment of the cells, which showed the same high fluorescence signal. Such problem was already encountered by previous researches.65
In the MCF-7 cell line, no increase in ROS was observed for ZnO and Zn(CUR)O-C compared to the control. On the other hand, the results showed a massive production of ROS when cells were treated with Zn(CUR)O-A and Zn(CUR)O-B. Similar results were observed for MDA-MB-231. The evaluation of ROS production showed an overall increase in cell cultures treated with Zn(CUR)O particles with higher concentrations of CUR. This was associated with an overall increased toxicity towards the treated cancer cell lines. Furthermore, such results are consistent with the reported narrowing of band gaps compared to pure ZnO NPs, although further analyses are required to support such a hypothesis.
Footnote |
† These authors contributed equally. |
This journal is © The Royal Society of Chemistry 2023 |