Issue 2, 2024

Development of an ic-ELISA and immunochromatographic assay strip for the rapid detection of chloridazon in oranges and celery

Abstract

Chloridazon (CLZ) is a selective herbicide used in the control of annual broadleaf weeds. The misuse or abuse of CLZ may result in the accumulation of CLZ in crops and water, which can pose a risk to human health. In this study, a hapten of CLZ with three carbon spacer arms was designed and a highly sensitive and specific antibody against CLZ was prepared with a half-maximal inhibitory concentration of 0.630 ng mL−1 and a linear range of 0.181–2.195 ng mL−1.Based on this antibody, we developed an immunochromatographic assay (ICA) strip for the detection of CLZ in oranges and celery. Under optimized conditions, the visual limit of detection was 2 ng mL−1 and 10 ng mL−1 in oranges and celery, respectively, and the cut-off value was 50 ng mL−1. In CLZ-spiked samples and the recovery test, the results of the ICA strip were consistent with those of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). Therefore, the ICA strip developed in our study represents an efficient and reliable method for the rapid screening of CLZ in oranges and celery.

Graphical abstract: Development of an ic-ELISA and immunochromatographic assay strip for the rapid detection of chloridazon in oranges and celery

Supplementary files

Article information

Article type
Paper
Submitted
17 Oct 2023
Accepted
20 Nov 2023
First published
04 Dec 2023

Analyst, 2024,149, 467-474

Development of an ic-ELISA and immunochromatographic assay strip for the rapid detection of chloridazon in oranges and celery

Q. Zhang, A. Wu, J. Li, L. Liu, H. Kuang, C. Xu and L. Guo, Analyst, 2024, 149, 467 DOI: 10.1039/D3AN01785K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements