Replacing the active site Cys-123 of dienelactone hydrolase with Ser completely changes the catalysis displayed by the protein, from hydrolysis of the substrate E- and Z-dienelactones to maleylacetate by the native enzyme, to interconversion of the substrates by the mutant, dienelactone isomerase.
Site-directed mutagenesis of dienelactone hydrolase produces dienelactone isomerase
I. Walker, C. J. Easton and D. L. Ollis, Chem. Commun., 2000, 671 DOI: 10.1039/B000365O
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