Issue 33, 2011
From the journal:

Chemical Communications

AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters†

Delphine Sicard,a   Samy Cecioni,bc   Maksym Iazykov,a   Yann Chevolot,a   Susan E. Matthews,d   Jean-Pierre Praly,b   Eliane Souteyrand,a   Anne Imberty,c   Sébastien Vidal*b  and  Magali Phaner-Goutorbe*a  

* Corresponding authors

a Université de Lyon, Institut des Nanotechnologies de Lyon (INL), UMR CNRS 5270, site Ecole Centrale de Lyon, 36, avenue Guy de Collongue, 69134 Ecully cedex, France
E-mail: magali.phaner@ec-lyon.fr

b Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR 5246), Laboratoire de Chimie Organique 2 – Glycochimie, CNRS, Université de Lyon, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
E-mail: sebastien.vidal@univ-lyon1.fr

c CERMAV – CNRS, UPR5301, affiliated with Université Joseph Fourier and ICMG, BP 53, Grenoble, France

d University of East Anglia, School of Pharmacy, Norwich, UK

Abstract

Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

Graphical abstract: AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

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Article information

DOI
https://doi.org/10.1039/C1CC13097H
Article type
Communication
Submitted
26 May 2011
Accepted
13 Jul 2011
First published
25 Jul 2011

Chem. Commun., 2011,47, 9483-9485

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AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

D. Sicard, S. Cecioni, M. Iazykov, Y. Chevolot, S. E. Matthews, J. Praly, E. Souteyrand, A. Imberty, S. Vidal and M. Phaner-Goutorbe, Chem. Commun., 2011, 47, 9483 DOI: 10.1039/C1CC13097H

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