Issue 47, 2011
From the journal:

Chemical Communications

A novel fluorescent probe for NAD-consuming enzymes†

Giulia Pergolizzi,abcd   Julea N. Butt,c   Richard P. Bowaterd  and  Gerd K. Wagner*b  

* Corresponding authors

a School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, UK

b King's College London, School of Biomedical Sciences, Institute of Pharmaceutical Science, Franklin-Wilkins Building, 150 Stamford Street, London, UK
E-mail: gerd.wagner@kcl.ac.uk
Fax: +44 (0)20 7848 4045
Tel: +44 (0)20 7848 4747

c School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, UK

d School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, UK

Abstract

A novel, fluorescent NAD derivative is processed as substrate by three different NAD-consuming enzymes. The new probe has been used to monitor enzymatic activity in a continuous format by changes in fluorescence and, in one case, to directly visualize alternative reaction pathways.

Graphical abstract: A novel fluorescent probe for NAD-consuming enzymes

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Article information

DOI
https://doi.org/10.1039/C1CC15499K
Article type
Communication
Submitted
05 Sep 2011
Accepted
17 Oct 2011
First published
31 Oct 2011

Chem. Commun., 2011,47, 12655-12657

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A novel fluorescent probe for NAD-consuming enzymes

G. Pergolizzi, J. N. Butt, R. P. Bowater and G. K. Wagner, Chem. Commun., 2011, 47, 12655 DOI: 10.1039/C1CC15499K

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